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Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control
Published on: March 30, 2015
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Overview and Future Perspectives of Microfluidic Digital Recombinase Polymerase Amplification (dRPA)
Ning Zhang1, Chao Li1, Xuechen Dou1
1Institute of Medical Support Technology, Academy of Military Science, Tianjin, China.
Critical Reviews in Analytical Chemistry
|February 24, 2022
Summary
Digital recombinase polymerase amplification (dRPA) quantifies nucleic acids using microfluidic systems. This review classifies microfluidic dRPA systems, analyzing their technologies and limitations for future development.
Area of Science:
- Molecular Biology
- Biotechnology
- Analytical Chemistry
Background:
- Digital recombinase polymerase amplification (dRPA) offers sensitive nucleic acid quantification.
- Microfluidics enhances dRPA for high-throughput, low-resource applications.
- Accurate nucleic acid quantification is crucial in diagnostics and research.
Purpose of the Study:
- To classify and review microfluidic dRPA systems developed over the last decade.
- To analyze key technologies and address limitations in microfluidic dRPA.
- To provide strategies for future development of these systems.
Main Methods:
- Literature review and classification of microfluidic dRPA systems.
- Analysis of chip preparation, segmentation, microfluidic control, and statistical methods.
- Identification of challenges such as evaporation, contamination, and manual operation.
Main Results:
- Microfluidic dRPA systems demonstrate high sensitivity, specificity, and throughput.
- Key technologies and limitations of various microfluidic dRPA platforms were identified.
- Strategies for preventing evaporation, contamination, and improving automation were discussed.
Conclusions:
- Microfluidic dRPA is a powerful tool for absolute nucleic acid quantification.
- Addressing current limitations will further enhance the utility and adoption of these systems.
- This review provides a roadmap for advancing microfluidic dRPA technology.
