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Control of Cell Geometry through Infrared Laser Assisted Micropatterning
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Novel Quick Cell Patterning Using Light-Responsive Gas-Generating Polymer and Fluorescence Microscope.

Hidetaka Ueno1, Yoshinori Akagi2, Shohei Yamamura1

  • 1Health and Medical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2217-14 Hayashi-cho, Takamatsu 761-0395, Kagawa, Japan.

Micromachines
|February 25, 2022
PubMed
Summary

A novel light-responsive gas-generating polymer enables precise cell patterning. This method rapidly releases cells from substrates using nitrogen gas bubbles, offering high accuracy and stability for various cell types and coatings.

Keywords:
cell chipscell microarraycell patterningfunctional polymerslab-on-a-chiplight-responsive gas-generating polymer

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Area of Science:

  • Biomaterials Science
  • Cell Biology
  • Microfabrication

Background:

  • Conventional cell patterning relies on surface properties like hydrophilicity/hydrophobicity or chemical coatings, leading to variable adhesion strength.
  • Existing methods often lack versatility and stability, being sensitive to substrate conditions, cell types, and extracellular matrix (ECM) components.
  • There is a need for improved cell patterning techniques that are accurate, stable, and broadly applicable.

Purpose of the Study:

  • To develop a new, versatile, and stable cell patterning method.
  • To utilize a light-responsive gas-generating polymer (LGP) for non-damaging cell release.
  • To demonstrate the efficacy of this method using a conventional fluorescence microscope.

Main Methods:

  • Fabrication of a polystyrene (PS) microarray chip with concave patterns, incorporating the light-responsive gas-generating polymer (LGP).
  • Seeding of HeLa cells onto LGP-implanted chips coated with different ECMs (fibronectin, collagen, poly-D-lysine).
  • Induction of nitrogen gas bubble generation from LGP using excitation light for cell release.

Main Results:

  • Successful release of HeLa cells from the LGP-patterned substrate without apparent cell damage.
  • Achieved high pattern accuracy with an error of 8.81 ± 4.24 μm, which is less than single-cell size.
  • Demonstrated scalability to millimeter-scale patterns and rapid patterning (<30 seconds).

Conclusions:

  • The proposed LGP-based method offers a simple, rapid, and highly accurate approach to cell patterning.
  • The technique exhibits excellent scalability and versatility, unaffected by cell type or ECM coating.
  • This light-responsive system provides a stable and non-damaging platform for advanced cell patterning applications.