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Updated: Oct 2, 2025
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Localization of SUMO-modified Proteins Using Fluorescent Sumo-trapping Proteins
Published on: April 27, 2019
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Ubiquitin and SUMO as timers during DNA replication
Rodrigo Martín-Rufo1, Guillermo de la Vega-Barranco1, Emilio Lecona1
1Chromatin, Cancer and the Ubiquitin System lab, Centre for Molecular Biology Severo Ochoa (CBMSO, CSIC-UAM), Department of Genome Dynamics and Function, Madrid 28049, Spain.
Seminars in Cell & Developmental Biology
|February 25, 2022
Summary
Ubiquitin and SUMO modifications, along with VCP/p97 ATPase, act as molecular timers crucial for DNA replication. This coordinated action ensures accurate DNA copying and maintains genome stability by regulating replication factors.
Area of Science:
- Molecular Biology
- Genetics
- Biochemistry
Background:
- DNA replication is prone to mutations and genomic alterations during cell division.
- Temporal regulation of DNA replication is essential for genomic stability.
- Post-translational modifications like ubiquitination and SUMOylation regulate protein function.
Purpose of the Study:
- To analyze the mechanisms of ubiquitin/SUMO and VCP/p97 in DNA replication.
- To investigate the role of these systems in establishing molecular timers.
- To understand their relevance in maintaining genome stability.
Main Methods:
- Analysis of post-translational modifications (ubiquitin/SUMO).
- Study of AAA+ ATPase VCP/p97 function in chromatin.
- Investigating the regulation of DNA replication factors.
Main Results:
- Ubiquitin/SUMO system and VCP/p97 coordinate DNA replication steps.
- These systems control activation, loading, and stabilization of replication factors.
- They establish molecular timers throughout the DNA replication process.
Conclusions:
- Ubiquitin/SUMO modifications and VCP/p97 are key regulators of DNA replication timing.
- These PTMs act as molecular watches from origin recognition to replisome disassembly.
- Their concerted action is vital for maintaining genome stability during replication.
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