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Related Concept Videos

DNA Topoisomerases02:02

DNA Topoisomerases

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Topoisomerases are enzymes that relax overwound DNA molecules during various cell processes, including DNA replication and transcription. These enzymes regulate positive and negative DNA supercoiling without changing the nucleotide sequence. DNA overwinding in a clockwise direction results in positively supercoiled DNA, whereas underwinding in a counterclockwise direction produces negatively supercoiled DNA.
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The DNA Replication Fork01:02

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An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
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DNA as a Genetic Template02:05

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Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...
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During replication, the complementary strands in double-stranded DNA are synthesized at different rates. Replication first begins on the leading strand. Replication starts later, occurs more slowly, and proceeds discontinuously on the lagging strand.
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Folding and Characterization of a Bio-responsive Robot from DNA Origami
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Folding and Characterization of a Bio-responsive Robot from DNA Origami

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Topogami: Topologically Linked DNA Origami.

Yusuke Sakai1, Gerrit D Wilkens1,2, Karol Wolski3

  • 1Bionanoscience and Biochemistry Laboratory, Malopolska Centre of Biotechnology, Jagiellonian University, Gronostajowa 7A, 30-387 Krakow, Poland.

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Researchers developed a new method to create linked DNA origami structures. This DNA nanotechnology breakthrough enables the production of complex molecular machines and motors using a simple one-pot protocol.

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Area of Science:

  • Nanotechnology
  • Molecular Biology
  • Supramolecular Chemistry

Background:

  • DNA origami is a versatile nanotechnology for creating nanoscale structures.
  • Topologically linked molecules, like catenanes, are promising for molecular machines.
  • A general method for producing topologically linked DNA origami was previously unavailable.

Purpose of the Study:

  • To establish a general method for producing topologically linked DNA origami structures.
  • To utilize catenated single-stranded DNA circles as a universal scaffold.
  • To enable the arbitrary design of individual linked structures within catenanes.

Main Methods:

  • Production of catenated single-stranded DNA circles.
  • Utilizing these circles as a universal scaffold for DNA origami assembly.
  • Employing a simple one-pot annealing protocol for structure formation.

Main Results:

  • Successful production of catenated single-stranded DNA circles.
  • Demonstration of these circles as a universal scaffold for linked DNA origami.
  • Assembly of topologically linked DNA origami structures with arbitrary designs.

Conclusions:

  • A general method for producing topologically linked DNA origami has been established.
  • This approach allows for the creation of complex, arbitrary linked nanostructures.
  • The developed method simplifies the production of DNA-based molecular machines and motors.