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Fat necrosis in human acute pancreatitis. An immunohistological study
Acta Pathologica, Microbiologica, Et Immunologica Scandinavica. Section A, Pathology
|March 1, 1986
Summary
In acute pancreatitis, phospholipase A2 and colipase, alongside lipase, contribute to fat necrosis development in pancreatic and surrounding adipose tissues. Mammary fat necrosis lacks these pancreatic enzymes.
Area of Science:
- Gastroenterology and Hepatology
- Surgical Pathology
- Biochemistry
Background:
- Fat necrosis is a pathological condition affecting adipose tissue.
- Acute pancreatitis involves inflammation and potential necrosis of pancreatic tissue.
- Mammary fat necrosis is a distinct condition occurring in breast adipose tissue.
Purpose of the Study:
- To investigate the localization and role of phospholipase A2, lipase, and colipase in fat necrosis.
- To differentiate the enzymatic mechanisms involved in pancreatic fat necrosis versus mammary fat necrosis.
Main Methods:
- Immunohistochemistry using the peroxidase-antiperoxidase method.
- Analysis of paraffin-embedded tissue sections from patients with acute pancreatitis and mammary fat necrosis.
Main Results:
- Phospholipase A2, lipase, and colipase immunoreactivity were detected at the border of fat necrosis in pancreatic and associated adipose tissues.
- Mammary fat necrosis tissues showed no reaction for these pancreatic enzymes.
- The findings suggest a role for these enzymes in the pathogenesis of fat necrosis in pancreatitis.
Conclusions:
- Phospholipase A2 and colipase, in addition to lipase, are implicated in the development of fat necrosis associated with acute pancreatitis.
- Pancreatic lipolytic enzymes are absent in mammary fat necrosis, indicating different etiological pathways.