Related Experiment Video
Updated: Oct 2, 2025
08:34
Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis
Published on: June 3, 2016
15.3K
Candidate genes responsible for lipid droplets formation during adipogenesis simultaneously affect osteoblastogenesis
1Jiangxi Provincial Key Laboratory of Systems Biomedicine, Jiujiang University, 17 Lufeng Road, Jiujiang 332000, China. yixia@mail.ecust.edu.cn.
Folia Histochemica Et Cytobiologica
|February 25, 2022
Summary
Gene expression during adipogenesis was profiled, revealing key genes in lipid droplet formation. APOB and METTL7A were found to influence both adipogenesis and osteoblastogenesis, impacting cell fate.
Area of Science:
- Cell Biology
- Molecular Biology
- Stem Cell Biology
Background:
- Cellular lipid storage is crucial for health, balancing lipid intake and degradation to regulate lipid droplets.
- While lipid droplet accumulation in adipocytes is known, gene expression and function during adipogenesis and osteoblastogenesis are not well understood.
Purpose of the Study:
- To profile gene expression during lipid droplet formation in adipogenesis.
- To investigate the function of candidate genes in adipogenesis and osteoblastogenesis.
Main Methods:
- RNA sequencing (RNA-Seq) was used to profile gene expression in human mesenchymal stem cells (hMSCs) during adipogenesis.
- RNA interference (RNAi) and lentivirus transfection assays were employed to study gene function.
- Oil Red, Alizarin Red, and alkaline phosphatase (ALPL) staining, along with quantitative real-time PCR (qRT-PCR), were used for phenotypic and transcriptional analysis.
Main Results:
- Eleven differentially upregulated genes related to lipid droplet formation were identified during adipogenesis.
- APOB inhibition impaired adipogenesis and weakened osteoblastogenesis.
- METTL7A facilitated adipogenesis with a negligible effect on osteoblastogenesis.
Conclusions:
- Gene expression profiling provides insights into molecular switches governing hMSC fate determination.
- Identified genes serve as potential study targets for metabolic diseases related to fat metabolism.
More Related Videos
09:41Measuring the Rate of Lipolysis in Ex Vivo Murine Adipose Tissue and Primary Preadipocytes Differentiated In Vitro
Published on: March 17, 2023
3.1K
06:08Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator
Published on: May 19, 2023
2.4K
Related Concept Videos
02:23Master Transcription Regulators
7.2K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.2K
01:58Cell Specific Gene Expression
14.1K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
14.1K
01:25Lipid Catabolism
214
Triglycerides serve as crucial long-term energy storage molecules in microorganisms, providing a dense source of metabolic energy. Their breakdown is mediated by lipases, which hydrolyze triglycerides into glycerol and free fatty acids. Each of these components follows distinct metabolic pathways, ultimately contributing to ATP synthesis and cellular energy homeostasis.Glycerol MetabolismGlycerol, released from triglyceride hydrolysis, is phosphorylated by glycerol kinase to form...
214
02:54Canonical Wnt Signaling Pathway
9.1K
The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...
9.1K
01:28Overview of Fatty Acid Metabolism
31.9K
Lipids also are sources of energy that power cellular processes. Like carbohydrates, lipids are composed of carbon, hydrogen, and oxygen, but these atoms are arranged differently. Most lipids are nonpolar and hydrophobic. Major types include fats and oils, waxes, phospholipids, and steroids.
Fatty acids are catabolized in a process called beta-oxidation, which takes place in the matrix of the mitochondria and converts their fatty acid chains into two-carbon units of acetyl groups. The acetyl...
Fatty acids are catabolized in a process called beta-oxidation, which takes place in the matrix of the mitochondria and converts their fatty acid chains into two-carbon units of acetyl groups. The acetyl...
31.9K
01:17Hormones and Bone Tissue
3.0K
The endocrine system produces and secretes hormones, which interact with the skeletal system. These hormones control bone growth, maintain bone once it is formed, and remodel it.
Hormones That Influence Osteoblasts and/or Maintain the Matrix
Several hormones are necessary for controlling bone growth and maintaining the bone matrix. The pituitary gland secretes growth hormone (GH), which, as its name implies, controls bone growth. This happens in several ways: first, it triggers chondrocyte...
Hormones That Influence Osteoblasts and/or Maintain the Matrix
Several hormones are necessary for controlling bone growth and maintaining the bone matrix. The pituitary gland secretes growth hormone (GH), which, as its name implies, controls bone growth. This happens in several ways: first, it triggers chondrocyte...
3.0K