Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Assessing water matrix influence and toxicity reduction of crystal violet and reactive black 5 dyes after cold plasma-driven degradation.

Scientific reports·2026

Same author

Sulfur Ylide-Mediated Cascade Transformations to Access Cyclopropylallocolchicinoids with Potent Antimitotic Activity.

JACS Au·2026

Same author

Quality and associated factors of guideline-concordant primary care referral for urinary incontinence and pelvic organ prolapse: A single centre retrospective observational study.

European journal of obstetrics, gynecology, and reproductive biology·2026

Same author

Emerging Urinary Biomarkers for Diagnosis, Phenotyping, and Treatment Monitoring in Female Overactive Bladder: A Systematic Review.

International urogynecology journal·2026

Same author

Myeloid AEG-1/MTDH drives inflammation and hepatocellular dysfunction in diet-induced steatohepatitis.

The Journal of biological chemistry·2025

Same author

Gene Therapy Strategies for Hepatocellular Carcinoma (HCC): Current Landscape and Future Directions.

Cancers·2025

Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Oct 2, 2025

Author Spotlight: Developing a Reproducible Method for Isolating Bone Marrow-Derived Macrophages from Neonatal Mice to Advance Early Life Immune Responses

Author Spotlight: Developing a Reproducible Method for Isolating Bone Marrow-Derived Macrophages from Neonatal Mice to Advance Early Life Immune Responses

Published on: May 24, 2024

Mouse Bone Marrow Cell Isolation and Macrophage Differentiation.

Rachel Mendoza¹, Indranil Banerjee¹, Debashri Manna¹

¹Department of Human and Molecular Genetics, Virginia Commonwealth University, Richmond, VA, USA.

Methods in Molecular Biology (Clifton, N.J.)

|February 25, 2022

Summary

This summary is machine-generated.

This study details a protocol for isolating mouse bone marrow cells and differentiating them into macrophages. These bone marrow-derived macrophages (BMDMs) are crucial for studying nonalcoholic steatohepatitis (NASH) development and progression.

Keywords:

Bone marrow cell F4/80 L929 cells Macrophage Macrophage colony-stimulating factor (M-CSF)Nonalcoholic steatohepatitis

More Related Videos

Isolation and Culture of Bone Marrow-Derived Macrophages from Mice

Isolation and Culture of Bone Marrow-Derived Macrophages from Mice

Published on: June 23, 2023

Bone Marrow-derived Macrophage Production

Bone Marrow-derived Macrophage Production

Published on: November 22, 2013

Related Experiment Videos

Last Updated: Oct 2, 2025

Author Spotlight: Developing a Reproducible Method for Isolating Bone Marrow-Derived Macrophages from Neonatal Mice to Advance Early Life Immune Responses

Author Spotlight: Developing a Reproducible Method for Isolating Bone Marrow-Derived Macrophages from Neonatal Mice to Advance Early Life Immune Responses

Published on: May 24, 2024

Isolation and Culture of Bone Marrow-Derived Macrophages from Mice

Isolation and Culture of Bone Marrow-Derived Macrophages from Mice

Published on: June 23, 2023

Bone Marrow-derived Macrophage Production

Bone Marrow-derived Macrophage Production

Published on: November 22, 2013

Area of Science:

Immunology
Hepatology
Cell Biology

Background:

Obesity incidence correlates with rising nonalcoholic steatohepatitis (NASH) cases.
Monocyte-derived macrophages contribute to NASH-related liver inflammation and fibrosis.
Adipose tissue macrophages (ATMs) promote lipolysis, releasing free fatty acids that induce liver steatosis.

Purpose of the Study:

To establish a reliable protocol for generating bone marrow-derived macrophages (BMDMs) in vitro.
To provide an accessible ex vivo model for investigating NASH pathogenesis.
To facilitate studies on macrophage roles in NASH, including cross-talk with hepatocytes and adipocytes.

Main Methods:

Isolation of primary mouse bone marrow cells.
In vitro differentiation of macrophages using macrophage colony-stimulating factor (M-CSF).
Confirmation of differentiation via F4/80 surface antigen immunofluorescence.

Main Results:

A reproducible protocol for mouse bone marrow-derived macrophage (BMDM) generation was established.
The differentiation protocol utilizes M-CSF, with options for conditioned L929 cell medium or purified M-CSF.
Immunofluorescence confirmed successful differentiation and expression of the macrophage marker F4/80.

Conclusions:

Bone marrow-derived macrophages (BMDMs) are essential for studying NASH pathogenesis.
The described protocol provides a valuable tool for NASH research.
BMDMs enable investigations into complex cellular interactions driving NASH progression.