Light stimulation strategy for promoting bio-hydrogen production: Microbial community, metabolic pathway and...

Light stimulation strategy for promoting bio-H₂ production was firstly investigated with incandescent light. The light condition controlled by photon number (N_R, 0.63 × 10⁴-6.25 × 10⁴ μmol/(day∙L)) was applied to stimulate H₂ fermentation process. The optimal N_R of 3.75 × 10⁴ μmol/(day∙L) contributed to 1.4 folds H₂ yield of the dark reactor and promoted efficient H₂ producing pathway (acetate and nicotinamide adenine dinucleotide pathway) with increased microbial activities. Furthermore, the effect of light stimulation on microbial community was identified. Fervidobacterium, Coprothermobacter and OPB95 were the dominant genera that could be activated by light stimulation for promoting acetate pathway and contribute to higher H₂ production. Moreover, long-term operation showed more stable and higher H₂ production of light stimulated bioreactor than the dark one, which resulted from the light stimulated metabolic pathway, increased sludge conductance and promoted microbial immobilization. This novel light stimulation strategy is promising for future application on promoting bio-H₂ production.