Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Maintenance of the ES Cell State01:14

Maintenance of the ES Cell State

2.3K
The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
2.3K
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

2.3K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.3K
Chromatin Modification in iPS Cells01:32

Chromatin Modification in iPS Cells

2.0K
Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
2.0K
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

24.5K
Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
24.5K
Combinatorial Gene Control02:33

Combinatorial Gene Control

8.5K
Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
8.5K
Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

1.9K
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
1.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Spin-Boson Mapping of the Quantum Approximate Optimization Algorithm.

Physical review letters·2026
Same author

Systematic Review and Meta-Analysis Examining the Effect of Upregulation and Inhibition of Proprotein Convertase Subtilisin/Kexin Type 9 in Mouse Models of Abdominal Aortic Aneurysm.

Arteriosclerosis, thrombosis, and vascular biology·2026
Same author

Predicting human mRNA isoform levels from site-specific splicing kinetics <i>in silico</i>.

bioRxiv : the preprint server for biology·2026
Same author

DNA-damage dependent interaction of Orc6 to SMARCA1 in S-phase modulates chromatin remodeling.

Nucleic acids research·2026
Same author

Polycomb-mediated 3D-genome organization controls replication timing.

Science advances·2026
Same author

Dual inhibition strategy against EGFR utilizing quercetin and 5-fluorouracil: A computational analysis for oral cancer treatment.

Computational biology and chemistry·2026

Related Experiment Video

Updated: Oct 2, 2025

A Simple Method to Identify Kinases That Regulate Embryonic Stem Cell Pluripotency by High-throughput Inhibitor Screening
07:18

A Simple Method to Identify Kinases That Regulate Embryonic Stem Cell Pluripotency by High-throughput Inhibitor Screening

Published on: May 12, 2017

6.6K

BEND3 safeguards pluripotency by repressing differentiation-associated genes.

Fredy Kurniawan1, Neha Chetlangia1, Mohammad Kamran1

  • 1Department of Cell and Developmental Biology, School of Molecular and Cellular Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801.

Proceedings of the National Academy of Sciences of the United States of America
|February 26, 2022
PubMed
Summary

BEN domain-containing protein 3 (BEND3) acts as a transcriptional repressor, crucial for maintaining pluripotency. Its removal triggers differentiation, highlighting BEND3

Keywords:
BEND3differentiationp21promotertranscription repression

More Related Videos

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal
08:01

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal

Published on: May 30, 2012

10.6K
A Two-Step Strategy that Combines Epigenetic Modification and Biomechanical Cues to Generate Mammalian Pluripotent Cells
08:01

A Two-Step Strategy that Combines Epigenetic Modification and Biomechanical Cues to Generate Mammalian Pluripotent Cells

Published on: August 29, 2020

2.4K

Related Experiment Videos

Last Updated: Oct 2, 2025

A Simple Method to Identify Kinases That Regulate Embryonic Stem Cell Pluripotency by High-throughput Inhibitor Screening
07:18

A Simple Method to Identify Kinases That Regulate Embryonic Stem Cell Pluripotency by High-throughput Inhibitor Screening

Published on: May 12, 2017

6.6K
Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal
08:01

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal

Published on: May 30, 2012

10.6K
A Two-Step Strategy that Combines Epigenetic Modification and Biomechanical Cues to Generate Mammalian Pluripotent Cells
08:01

A Two-Step Strategy that Combines Epigenetic Modification and Biomechanical Cues to Generate Mammalian Pluripotent Cells

Published on: August 29, 2020

2.4K

Area of Science:

  • * Molecular Biology
  • * Gene Regulation
  • * Developmental Biology

Background:

  • * BEN domain-containing proteins regulate gene expression, but their chromatin function is unclear.
  • * BEND3, a quadruple BEN domain protein, associates with heterochromatin and represses transcription.
  • * BEND3 is highly expressed in pluripotent cells and downregulated during differentiation.

Purpose of the Study:

  • * To elucidate the molecular mechanisms by which BEND3 regulates chromatin function and transcription.
  • * To investigate the role of BEND3 in maintaining pluripotency and preventing differentiation.
  • * To understand BEND3's impact on differentiation-associated gene expression and cell cycle regulators.

Main Methods:

  • * Analysis of BEND3 expression during cell differentiation.
  • * Investigating the effect of BEND3 depletion on gene expression signatures.
  • * Chromatin immunoprecipitation to assess BEND3 binding and H3K27me3 enrichment at target gene promoters.

Main Results:

  • * BEND3 is highly expressed in pluripotent cells and downregulated upon differentiation induction.
  • * Depletion of BEND3 in pluripotent cells leads to upregulation of differentiation-associated genes.
  • * BEND3 binds to promoters of differentiation factors and cell cycle regulators (e.g., CDKN1A), enhancing H3K27me3 and repressing expression.

Conclusions:

  • * BEND3 functions as a transcriptional repressor essential for maintaining pluripotency.
  • * BEND3-mediated repression prevents premature differentiation by suppressing differentiation-associated genes.
  • * BEND3 plays a critical role in normal development by regulating gene expression and chromatin state.