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A putative cap binding protein and the methyl phosphate capping enzyme Bin3/MePCE function in telomerase biogenesis
Diego J Páez-Moscoso1,2,3, David V Ho1,2, Lili Pan1
1Faculty of Biology, Johannes Gutenberg University, 55099, Mainz, Germany.
Nature Communications
|February 26, 2022
Summary
Two new proteins, Thc1 and Bmc1, were discovered to be essential for telomerase assembly and function in fission yeast. They work with Pof8 to ensure telomere length maintenance and overall telomerase activity.
Area of Science:
- Cell Biology
- Molecular Biology
- Biochemistry
Background:
- Telomerase is a ribonucleoprotein enzyme essential for maintaining telomere length.
- Telomerase biogenesis involves the assembly of multiple protein and RNA subunits.
- Pof8 is a known constitutive component of telomerase in fission yeast.
Purpose of the Study:
- To identify novel proteins involved in telomerase biogenesis.
- To elucidate the roles of these new proteins in telomerase assembly and function.
Main Methods:
- Affinity purification of Pof8.
- Co-immunoprecipitation assays.
- Functional assays for telomerase activity and telomere length maintenance.
Main Results:
- Two previously uncharacterized proteins, Thc1 and Bmc1, were identified as Pof8-interacting partners.
- Thc1 and Bmc1 are crucial for telomerase ribonucleoprotein complex assembly.
- These proteins are required for wild-type telomerase activity and telomere length maintenance.
Conclusions:
- Thc1 and Bmc1, along with Pof8, form a complex that recognizes correctly folded telomerase RNA.
- This complex facilitates the recruitment of the Lsm2-8 complex and the catalytic subunit for functional telomerase assembly.
- These findings reveal new key players in the intricate process of telomerase biogenesis.
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