Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Co-activators and Co-repressors02:04

Co-activators and Co-repressors

7.7K
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
7.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Monitoring endosomal escape of fluorescent cell-penetrating peptides.

European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences·2025
Same author

No Evidence for Plasma Membrane Potential-Independent Cell Penetrating Peptide Direct Translocation.

Journal of peptide science : an official publication of the European Peptide Society·2025
Same author

Corrigendum to "Plasma membrane depolarization reveals endosomal escape incapacity of cell-penetrating peptides" [Eur. J. Pharm. Biopharm. 184 (2023) 13949].

European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V·2025
Same author

Repression motif in HSF1 regulated by phosphorylation.

Cellular signalling·2023
Same author

Plasma membrane depolarization reveals endosomal escape incapacity of cell-penetrating peptides.

European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V·2023
Same author

Senescence in a cell culture model for burn wounds.

Experimental and molecular pathology·2021
Same journal

Cloning and Functional Characterization of AhyAP-T65Lig, an ATP-Dependent DNA Ligase from Trabzonvirus AP-T65.

Molecular biotechnology·2026
Same journal

Overexpression of the ATP-Citrate Lyase Gene Enhances Ganoderic Acid Biosynthesis in Ganoderma lingzhi.

Molecular biotechnology·2026
Same journal

CRISPR/Cas9 Mediated Genome Editing for Enhancing Abiotic Stress Tolerance in Rice: An Omics Guided Perspective.

Molecular biotechnology·2026
Same journal

Functionalization of Chitosan with Silver Oxide and Berberis aristata for Bioactive Composites with In Vivo Wound Healing Evaluation.

Molecular biotechnology·2026
Same journal

Resilience in Tribolium Castaneum Through Antioxidant Priming Under Phosphine-Induced Oxidative Stress.

Molecular biotechnology·2026
Same journal

MicroRNA-181a-5p promotes papillary thyroid carcinoma progress via the PTEN/AKT pathway.

Molecular biotechnology·2026
See all related articles

Related Experiment Video

Updated: Oct 2, 2025

Expression Analysis of Mammalian Linker-histone Subtypes
14:40

Expression Analysis of Mammalian Linker-histone Subtypes

Published on: March 19, 2012

13.9K

Quantitative Comparison of HSF1 Activators.

Christoph Steurer1, Sarah Kerschbaum1, Christina Wegrostek1

  • 1Department of Applied Life Sciences, University of Applied Sciences, FH Campus Wien, Helmut-Qualtinger-Gasse 2, 1030, Vienna, Austria.

Molecular Biotechnology
|February 26, 2022
PubMed
Summary
This summary is machine-generated.

Activators of the heat shock response (HSR) pathway show varied effectiveness in clearing cellular aggregates. This study quantitatively compares HSR activators, revealing significant differences in potency and specificity, crucial for therapeutic development.

Keywords:
HSF1Heat shock responseLuciferase assayNeurodegenerative disease

More Related Videos

High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy
06:38

High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy

Published on: February 7, 2019

8.9K
Assays for Validating Histone Acetyltransferase Inhibitors
09:11

Assays for Validating Histone Acetyltransferase Inhibitors

Published on: August 6, 2020

6.7K

Related Experiment Videos

Last Updated: Oct 2, 2025

Expression Analysis of Mammalian Linker-histone Subtypes
14:40

Expression Analysis of Mammalian Linker-histone Subtypes

Published on: March 19, 2012

13.9K
High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy
06:38

High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy

Published on: February 7, 2019

8.9K
Assays for Validating Histone Acetyltransferase Inhibitors
09:11

Assays for Validating Histone Acetyltransferase Inhibitors

Published on: August 6, 2020

6.7K

Area of Science:

  • Cellular biology
  • Molecular mechanisms of disease
  • Drug discovery

Background:

  • The heat shock response (HSR) pathway is a vital cellular defense mechanism against proteotoxic stress, involving chaperones to maintain protein homeostasis.
  • Dysregulation of HSR is implicated in diseases like neurodegeneration and cancer, where chaperone overexpression is a therapeutic target.
  • Heat shock factor 1 (HSF1) is the master regulator of HSR, making its activators key targets for therapeutic intervention.

Purpose of the Study:

  • To quantitatively compare a wide range of published HSR pathway activators.
  • To assess activator potency and specificity using a novel HSF-responsive dual-luciferase reporter cell line.
  • To compare reporter assay results with endogenous heat shock protein expression.

Main Methods:

  • Development of a novel dual-luciferase reporter cell line specifically responsive to HSF activity.
  • Quantitative screening and comparison of diverse HSR pathway activators.
  • Parallel assessment of cell viability to determine drug specificity.
  • Comparison of reporter assay data with endogenous heat shock protein levels.

Main Results:

  • Significant variations in the intensity of HSR pathway activation were observed among different published activators.
  • Cell viability assays revealed considerable differences in the specificity of these drugs.
  • Discrepancies between the reporter assay and published data suggest potential tissue-specific effects of HSR activators.
  • Comparison with endogenous heat shock protein expression highlighted variability in pathway activation.

Conclusions:

  • The study underscores the heterogeneity in potency and specificity among known HSR activators.
  • Findings suggest that the efficacy of HSR activators may be context-dependent, potentially exhibiting tissue-specific differences.
  • The developed reporter system provides a valuable tool for quantitative comparison and discovery of novel HSR modulators.
  • Further investigation into tissue-specific regulation of HSF1 is warranted for targeted therapeutic strategies.