Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Evaluation of candidate RT-qPCR reference genes in the aging African turquoise killifish brain.

Aging brain·2025

Same author

Decellularized Extracellular Matrix Scaffolds to Engineer the Dormant Landscape of Microscopic Colorectal Cancer Liver Metastasis.

Advanced healthcare materials·2025

Same author

Gene expression detection methods in the African turquoise killifish brain.

Developmental dynamics : an official publication of the American Association of Anatomists·2025

Same author

Widefield frequency-domain autofluorescence lifetime imaging for detecting breast cancer in murine xenograft tumor tissues.

Journal of biomedical optics·2025

Same author

Seeing deep to map cell-biomaterial interactions <i>via</i> whole mount light sheet imaging and automated analytics in decellularized extracellular matrix models.

Biomaterials science·2025

Same author

One Wnt to lead them all: a Wnt1 primer.

Differentiation; research in biological diversity·2025

Same journal

Isolation of Mesenchymal Stem Cell-Derived Extracellular Vesicles.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Modeling Melanoma Immune Surveillance by CAR-T Cells in Human Skin Organoids.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Stepwise Optimization of a Matrigel-Based In Vitro Angiogenesis Assay for Reproducible and Quantifiable 2D-Tube Formation Using HUVECs.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Quantifying Mechanical Properties of Fresh Ovarian Tissue with Optical Brillouin Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

3D Chromatin Architecture During Early Development: New Methods and New Findings.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Metabolic Plasticity in Embryogenesis Throughout the Lens of NAD<sup></sup>.

Methods in molecular biology (Clifton, N.J.)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Oct 2, 2025

Intravital Imaging of Intraepithelial Lymphocytes in Murine Small Intestine

Intravital Imaging of Intraepithelial Lymphocytes in Murine Small Intestine

Published on: June 24, 2019

Quantifiable Intravital Light Sheet Microscopy.

Holly C Gibbs^1,2, Sreeja Sarasamma³, Oscar R Benavides³

¹Department of Biomedical Engineering, Texas A&M University, College Station, TX, USA. hgibbs@tamu.edu.

Methods in Molecular Biology (Clifton, N.J.)

|February 26, 2022

Summary

This summary is machine-generated.

We developed a novel mounting strategy for live imaging of zebrafish embryos, enabling high-quality visualization of cellular movements in the developing brain. This technique overcomes common challenges in zebrafish microscopy for developmental studies.

Keywords:

Bioimage analysis Light sheet fluorescence microscopy Midbrain–hindbrain boundary Zebrafish

More Related Videos

Long-term Intravital Immunofluorescence Imaging of Tissue Matrix Components with Epifluorescence and Two-photon Microscopy

Long-term Intravital Immunofluorescence Imaging of Tissue Matrix Components with Epifluorescence and Two-photon Microscopy

Published on: April 22, 2014

Visualization of Motor Axon Navigation and Quantification of Axon Arborization In Mouse Embryos Using Light Sheet Fluorescence Microscopy

Visualization of Motor Axon Navigation and Quantification of Axon Arborization In Mouse Embryos Using Light Sheet Fluorescence Microscopy

Published on: May 11, 2018

Related Experiment Videos

Last Updated: Oct 2, 2025

Intravital Imaging of Intraepithelial Lymphocytes in Murine Small Intestine

Intravital Imaging of Intraepithelial Lymphocytes in Murine Small Intestine

Published on: June 24, 2019

Long-term Intravital Immunofluorescence Imaging of Tissue Matrix Components with Epifluorescence and Two-photon Microscopy

Long-term Intravital Immunofluorescence Imaging of Tissue Matrix Components with Epifluorescence and Two-photon Microscopy

Published on: April 22, 2014

Visualization of Motor Axon Navigation and Quantification of Axon Arborization In Mouse Embryos Using Light Sheet Fluorescence Microscopy

Visualization of Motor Axon Navigation and Quantification of Axon Arborization In Mouse Embryos Using Light Sheet Fluorescence Microscopy

Published on: May 11, 2018

Area of Science:

Developmental Biology
Microscopy Techniques
Zebrafish Models

Background:

Live imaging of zebrafish embryos presents challenges including sample mounting, immobilization, and phototoxicity.
Poor image quality often hinders detailed cellular analysis in developing zebrafish.
Understanding cellular dynamics in the embryonic brain requires advanced imaging solutions.

Purpose of the Study:

To describe a mounting strategy for imaging the zebrafish midbrain-hindbrain boundary (MHB).
To establish parameters for semiautomatically tracking cellular movements in the embryonic brain using light sheet fluorescence microscopy (LSFM).
To improve image quality for detailed analysis of zebrafish embryonic development.

Main Methods:

Developed a specific sample mounting technique for zebrafish embryos.
Utilized light sheet fluorescence microscopy (LSFM) for live imaging.
Implemented pilot experiments to define parameters for semiautomatic cellular tracking.

Main Results:

Successfully imaged zebrafish embryos with a focus on the midbrain-hindbrain boundary (MHB).
Achieved sufficient image quality for detailed cellular analysis.
Developed parameters for semiautomatically tracking cellular movements during zebrafish segmentation.

Conclusions:

The described mounting strategy enhances live imaging of zebrafish embryos.
This method facilitates detailed analysis of cellular dynamics in the developing zebrafish brain.
The approach provides a foundation for further studies on embryonic development using advanced microscopy.