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Updated: Oct 2, 2025

Intravital Imaging of Intraepithelial Lymphocytes in Murine Small Intestine

Intravital Imaging of Intraepithelial Lymphocytes in Murine Small Intestine

Published on: June 24, 2019

Quantifiable Intravital Light Sheet Microscopy.

Holly C Gibbs^1,2, Sreeja Sarasamma³, Oscar R Benavides³

¹Department of Biomedical Engineering, Texas A&M University, College Station, TX, USA. hgibbs@tamu.edu.

Methods in Molecular Biology (Clifton, N.J.)

|February 26, 2022

Summary

This summary is machine-generated.

We developed a novel mounting strategy for live imaging of zebrafish embryos, enabling high-quality visualization of cellular movements in the developing brain. This technique overcomes common challenges in zebrafish microscopy for developmental studies.

Keywords:

Bioimage analysis Light sheet fluorescence microscopy Midbrain–hindbrain boundary Zebrafish

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Visualization of Motor Axon Navigation and Quantification of Axon Arborization In Mouse Embryos Using Light Sheet Fluorescence Microscopy

Visualization of Motor Axon Navigation and Quantification of Axon Arborization In Mouse Embryos Using Light Sheet Fluorescence Microscopy

Published on: May 11, 2018

Area of Science:

Developmental Biology
Microscopy Techniques
Zebrafish Models

Background:

Live imaging of zebrafish embryos presents challenges including sample mounting, immobilization, and phototoxicity.
Poor image quality often hinders detailed cellular analysis in developing zebrafish.
Understanding cellular dynamics in the embryonic brain requires advanced imaging solutions.

Purpose of the Study:

To describe a mounting strategy for imaging the zebrafish midbrain-hindbrain boundary (MHB).
To establish parameters for semiautomatically tracking cellular movements in the embryonic brain using light sheet fluorescence microscopy (LSFM).
To improve image quality for detailed analysis of zebrafish embryonic development.

Main Methods:

Developed a specific sample mounting technique for zebrafish embryos.
Utilized light sheet fluorescence microscopy (LSFM) for live imaging.
Implemented pilot experiments to define parameters for semiautomatic cellular tracking.

Main Results:

Successfully imaged zebrafish embryos with a focus on the midbrain-hindbrain boundary (MHB).
Achieved sufficient image quality for detailed cellular analysis.
Developed parameters for semiautomatically tracking cellular movements during zebrafish segmentation.

Conclusions:

The described mounting strategy enhances live imaging of zebrafish embryos.
This method facilitates detailed analysis of cellular dynamics in the developing zebrafish brain.
The approach provides a foundation for further studies on embryonic development using advanced microscopy.