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Tissue Homogenization and Cell Lysis

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Tissue homogenization involves disintegrating tissue architecture and lysing cells, and is an early step in isolating and analyzing cellular components. The method used for homogenization depends on the sample type, the amount of sample available, the analyte to be obtained, and the sensitivity of the method. These methods are broadly classified as mechanical and non-mechanical methods.
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Updated: Oct 2, 2025

Optical Clearing of the Mouse Central Nervous System Using Passive CLARITY

Optical Clearing of the Mouse Central Nervous System Using Passive CLARITY

Published on: June 30, 2016

Hydrophobic and Hydrogel-Based Methods for Passive Tissue Clearing.

Frank L Jalufka¹, Sun Won Min¹, Madison E Platt¹

¹Department of Biology, Texas A&M University, College Station, TX, USA.

Methods in Molecular Biology (Clifton, N.J.)

|February 26, 2022

Summary

This summary is machine-generated.

Optical tissue clearing allows detailed 3D imaging of whole organs without sectioning. Two passive clearing methods, PACT and iDISCO+, are presented for immunofluorescence and lightsheet microscopy.

Keywords:

Lightsheet microscopy PACT Passive CLARITY technique Tissue clearing iDISCO+

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Last Updated: Oct 2, 2025

Optical Clearing of the Mouse Central Nervous System Using Passive CLARITY

Optical Clearing of the Mouse Central Nervous System Using Passive CLARITY

Published on: June 30, 2016

Novel Passive Clearing Methods for the Rapid Production of Optical Transparency in Whole CNS Tissue

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Published on: May 8, 2018

ACT-PRESTO: Biological Tissue Clearing and Immunolabeling Methods for Volume Imaging

ACT-PRESTO: Biological Tissue Clearing and Immunolabeling Methods for Volume Imaging

Published on: December 31, 2016

Area of Science:

Biomedical Imaging
Cell Biology
Histology

Background:

Physical tissue sectioning limits 3D visualization of organs.
Optical tissue clearing offers a non-destructive alternative for high-resolution imaging.
Integrating clearing with advanced microscopy is crucial for large-scale biological studies.

Purpose of the Study:

To demonstrate passive optical tissue clearing techniques compatible with immunofluorescence.
To evaluate PACT (passive clarity tissue clearing) and iDISCO+ for 3D imaging.
To enable visualization of cellular structures in whole organs using lightsheet microscopy.

Main Methods:

Two passive tissue clearing methods were employed: PACT (aqueous hydrogel-based) and iDISCO+ (organic solvent-based).
Techniques were optimized for compatibility with immunofluorescent staining.
Cleared tissues were imaged using lightsheet microscopy.

Main Results:

Both PACT and iDISCO+ successfully rendered whole organs transparent.
Immunofluorescence staining remained intact after clearing, allowing specific structure visualization.
High-resolution 3D images of cellular and subcellular details were acquired.

Conclusions:

PACT and iDISCO+ are effective passive tissue clearing methods for 3D imaging.
These techniques facilitate detailed analysis of tissue architecture and cellular distribution.
Optical clearing combined with lightsheet microscopy advances biological research by enabling comprehensive specimen analysis.