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Related Concept Videos

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Updated: Oct 2, 2025

Conducting Multiple Imaging Modes with One Fluorescence Microscope
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Nanometer-Scale Molecular Mapping by Super-resolution Fluorescence Microscopy.

Vito Mennella1, Zhen Liu2

  • 1MRC Toxicology Unit, School of Biological Sciences, University of Cambridge, Cambridge, UK. vm430@cam.ac.uk.

Methods in Molecular Biology (Clifton, N.J.)
|February 26, 2022
PubMed
Summary
This summary is machine-generated.

This study details a workflow for creating nanometer-scale maps of cellular structures. It focuses on using two super-resolution microscopy techniques: structured illumination microscopy (SIM) and stochastic optical reconstruction microscopy (STORM).

Keywords:
CentrosomeCiliaMacromolecular assembly organizationSIMSTORMSubdiffraction imagingSuper-resolution fluorescence microscopy

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Area of Science:

  • Cellular Biology
  • Biophysics
  • Microscopy

Background:

  • Understanding cellular function relies on knowing the structure of macromolecules and their organization in cellular components.
  • Super-resolution fluorescence microscopy allows for detailed visualization of nanoscale cellular structures.
  • Positional mapping of proteins in situ is crucial for studying cellular organization.

Purpose of the Study:

  • To present a detailed workflow for constructing nanometer-scale maps of cellular structures.
  • To highlight the application of two complementary super-resolution microscopy techniques for this purpose.

Main Methods:

  • Structured Illumination Microscopy (SIM)
  • Stochastic Optical Reconstruction Microscopy (STORM)
  • Workflow development for in situ protein mapping at the nanoscale.

Main Results:

  • Demonstration of a comprehensive workflow for nanoscale mapping.
  • Integration of SIM and STORM modalities for enhanced structural analysis.
  • Successful positional mapping of proteins within cellular assemblies.

Conclusions:

  • The presented workflow enables detailed nanometer-scale mapping of cellular organization.
  • Combining SIM and STORM provides complementary insights into macromolecular structures.
  • This approach advances the study of cellular function through precise structural elucidation.