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Protein-protein Interfaces

Protein-protein Interfaces

Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...

Protein Networks

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
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Protein-Protein Interfaces

Protein-Protein Interfaces

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Related Experiment Video

Updated: Oct 2, 2025

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay PCA in Living Cells

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay PCA in Living Cells

Published on: March 3, 2015

Exploring protein-protein interactions at the proteome level.

Hadeer Elhabashy¹, Felipe Merino², Vikram Alva²

¹Department of Protein Evolution, Max Planck Institute for Biology, Tübingen, Germany; Department of Computer Science, University of Tübingen, WSI/ZBIT, Sand 14, 72076 Tübingen, Germany; Institute for Bioinformatics and Medical Informatics, University of Tübingen, Sand 14, 72076 Tübingen, Germany.

Structure (London, England : 1993)

|February 27, 2022

Summary

This summary is machine-generated.

Understanding protein-protein interactions is crucial for molecular biology. This review surveys computational and experimental methods for characterizing these interactions, highlighting integrative approaches for improved accuracy.

Keywords:

high-throughput techniques interactome protein-protein interaction proteome

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Last Updated: Oct 2, 2025

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Area of Science:

Molecular Biology
Biochemistry
Proteomics

Background:

Proteins are fundamental to life processes and interact with other macromolecules.
Protein-protein interactions are the most common macromolecular interactions in living systems.
Characterizing these interactions is essential for a molecular understanding of biological systems.

Purpose of the Study:

To review computational and experimental techniques for protein-protein interaction characterization.
To discuss the potential of integrative approaches for improving interaction analysis.
To address the challenges in accurately characterizing protein-protein interactions.

Main Methods:

Survey of computational methods for protein-protein interaction identification.
Survey of experimental techniques for protein-protein interaction characterization.
Discussion of integrative strategies combining sequence and structure data.

Main Results:

Identification of various techniques for medium- to high-throughput characterization of protein-protein interactions.
Highlighting the importance of integrative approaches.
Acknowledging ongoing challenges in accuracy and resolution.

Conclusions:

Accurate characterization of protein-protein interactions remains a significant challenge in molecular biology.
Advancements in sequence analysis and structure prediction enhance integrative approaches.
Further development of both computational and experimental methods is needed.