Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Early Endosome: Endocytosis of Transferrin01:28

The Early Endosome: Endocytosis of Transferrin

3.5K
Essential proteins such as insulin or low-density lipoprotein (LDL) and micronutrients such as iron enter a eukaryotic cell through receptor-mediated endocytosis. Subsequently, the early endosomes fuse with the vesicles containing such receptor-ligand complexes and play a vital role in sorting the incoming ligands and receptors. While the ligands are either degraded inside the vesicle or released into the cytosol, their receptors are returned to the plasma membrane for further rounds of...
3.5K
ER Retrieval Pathway01:45

ER Retrieval Pathway

4.0K
In the secretory pathway, vesicles transport proteins from one cellular compartment to another in forward transport to deliver the protein to its correct location. Occasionally, misfolded proteins and incorrect proteins escape their original compartments, and a retrieval pathway is used to return the escaped proteins to their original compartment.
The ER uses many checkpoints to prevent the entry of incorrectly folded or a resident protein as cargo onto a transport vesicle. These mechanisms...
4.0K
Maturation of Endosomes01:28

Maturation of Endosomes

4.5K
The early endosome containing internalized molecules matures through transformations in its location, morphology, intraluminal pH, and membrane protein composition. Together, these changes result in a more acidic late endosome that contains multiple intraluminal vesicles; therefore, the late endosome is also called a multivesicular body (MVB).
Changes in location
The maturing endosome moves along microtubules from the periphery of the cell towards the perinuclear region. This movement of the...
4.5K
Animal Mitochondrial Genetics02:59

Animal Mitochondrial Genetics

8.2K
Among all the organelles in an animal cell, only mitochondria have their own independent genomes. Animal mitochondrial DNA is a double-stranded, closed-circular molecule with around 20,000 base pairs. Mitochondrial DNA is unique in that one of its two strands, the heavy, or H, -strand is guanine rich, whereas the complementary strand is cytosine rich and called the light, or L, -strand. Compared to nuclear DNA, mitochondrial DNA has a very low percentage of non-coding regions and is marked by...
8.2K
Inheritance of Chromatin Structures03:17

Inheritance of Chromatin Structures

6.7K
Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...
6.7K
Delivery Pathways to the Lysosome01:36

Delivery Pathways to the Lysosome

8.0K
Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
8.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Glutamine codon-driven translational readthrough reveals context-dependent stop codon decoding fidelity.

bioRxiv : the preprint server for biology·2026
Same author

Proteasome activator Blm10 maintains cellular proteostatic balance and gamete quality in budding yeast.

bioRxiv : the preprint server for biology·2026
Same author

Tom70-mediated mitochondria-nuclear envelope contacts regulate nuclear pore complex inheritance during gametogenesis.

bioRxiv : the preprint server for biology·2025
Same author

A microfluidics-based platform enables discovery of gametogenic rejuvenation factors in <i>Saccharomyces cerevisiae</i>.

bioRxiv : the preprint server for biology·2025
Same author

Meiotic resetting of the cellular Sod1 pool is driven by protein aggregation, degradation, and transient LUTI-mediated repression.

The Journal of cell biology·2023
Same author

Bidirectional promoter activity from expression cassettes can drive off-target repression of neighboring gene translation.

eLife·2022

Related Experiment Video

Updated: Oct 2, 2025

Manipulation of Ploidy in Caenorhabditis elegans
07:54

Manipulation of Ploidy in Caenorhabditis elegans

Published on: March 15, 2018

11.2K

Developmentally regulated selective autophagy determines ER inheritance by gametes.

George Maxwell Otto1, Gloria Ann Brar1

  • 1Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA.

Autophagy
|February 28, 2022
PubMed
Summary

Developmentally regulated reticulophagy, a form of selective autophagy, unexpectedly occurs during yeast meiosis. This process, driven by Atg40, removes specific endoplasmic reticulum components, preventing their inheritance by gametes.

Keywords:
Atg40ERphagyendoplasmic reticulumgametesmeiosisquality controlreticulophagy

More Related Videos

Combining Magnetic Sorting of Mother Cells and Fluctuation Tests to Analyze Genome Instability During Mitotic Cell Aging in Saccharomyces cerevisiae
11:08

Combining Magnetic Sorting of Mother Cells and Fluctuation Tests to Analyze Genome Instability During Mitotic Cell Aging in Saccharomyces cerevisiae

Published on: October 16, 2014

12.7K
Stable Isotope In-Vivo Labeling for Mass-Spectrometry Identification of Paternal Metabolites Transferred from Sperm to Oocyte During Fertilization
05:55

Stable Isotope In-Vivo Labeling for Mass-Spectrometry Identification of Paternal Metabolites Transferred from Sperm to Oocyte During Fertilization

Published on: June 17, 2025

412

Related Experiment Videos

Last Updated: Oct 2, 2025

Manipulation of Ploidy in Caenorhabditis elegans
07:54

Manipulation of Ploidy in Caenorhabditis elegans

Published on: March 15, 2018

11.2K
Combining Magnetic Sorting of Mother Cells and Fluctuation Tests to Analyze Genome Instability During Mitotic Cell Aging in Saccharomyces cerevisiae
11:08

Combining Magnetic Sorting of Mother Cells and Fluctuation Tests to Analyze Genome Instability During Mitotic Cell Aging in Saccharomyces cerevisiae

Published on: October 16, 2014

12.7K
Stable Isotope In-Vivo Labeling for Mass-Spectrometry Identification of Paternal Metabolites Transferred from Sperm to Oocyte During Fertilization
05:55

Stable Isotope In-Vivo Labeling for Mass-Spectrometry Identification of Paternal Metabolites Transferred from Sperm to Oocyte During Fertilization

Published on: June 17, 2025

412

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Autophagy Research

Background:

  • The endoplasmic reticulum (ER) is crucial for cellular functions like protein and metabolite processing.
  • ER homeostasis is maintained by quality control pathways, including reticulophagy (ER-selective autophagy), particularly under stress.
  • Reticulophagy's role in development was previously unidentified.

Purpose of the Study:

  • To investigate ER remodeling during meiosis in budding yeast.
  • To identify the mechanisms and roles of reticulophagy during this developmental process.

Main Methods:

  • Observation of ER morphology during meiosis in budding yeast.
  • Analysis of the role of the autophagy receptor Atg40 in developmentally regulated reticulophagy.
  • Investigating the fate of ER subpopulations during gamete formation.

Main Results:

  • Unexpected observation of developmentally regulated reticulophagy during budding yeast meiosis.
  • Reticulophagy was driven by the expression of the autophagy receptor Atg40.
  • This process coordinated with significant ER morphological rearrangement, including peripheral ER retraction.
  • Specific ER subpopulations were prevented from being inherited by gametes.

Conclusions:

  • A novel role for reticulophagy during cellular development (meiosis) has been identified.
  • Developmentally regulated reticulophagy, mediated by Atg40, plays a role in ER remodeling.
  • Meiotic reticulophagy likely functions as a quality control mechanism to prevent transmission of potentially harmful material to the next generation.