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Untargeted Liquid Chromatography-Mass Spectrometry-Based Metabolomics Analysis of Wheat Grain
Published on: March 13, 2020
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Integrated LC-MS and GC-MS-Based Metabolomics Reveal the Effects of Plant Competition on the Rye Metabolome
Hossein Hazrati1,2, Per Kudsk1, Ling Ding3
1Department of Agroecology, Aarhus University, Forsøgsvej 1, 4200 Slagelse, Denmark.
Journal of Agricultural and Food Chemistry
|March 1, 2022
Summary
Plant competition alters rye (Secale cereale) metabolome, affecting defense compounds like benzoxazinoids (BXs) and increasing allelochemicals in roots. Shoots showed elevated d-pyroglutamic acid, indicating a stress response.
Area of Science:
- Plant biology
- Metabolomics
- Plant biochemistry
Background:
- Plants compete for limited resources, triggering stress responses.
- Competition can induce metabolic alterations for resilience and defense.
Purpose of the Study:
- Investigate rye (Secale cereale) metabolome changes under varying competition densities.
- Elucidate rye's biochemical response to interspecific competition with legumes.
Main Methods:
- Cocultivation of rye with Austrian pea, hairy vetch, and Alexandrian clover at different densities.
- Global metabolic profiling using LC-QqQ-MS, LC-QTOF-MS, and GC-TOF-MS.
- Uni- and multivariate statistical analysis of metabolomic data.
Main Results:
- High competition reduced aglycone benzoxazinoids (BXs) and increased glycoside BXs in rye roots.
- Allelopathic compounds 4-hydroxybenzoic acid and uracil increased in roots under competition.
- D-pyroglutamic acid, a reactive oxygen species (ROS) elicitor, increased in rye shoots.
Conclusions:
- Plant competition significantly reshapes the rye metabolome.
- Metabolic shifts involve changes in defense compounds and the accumulation of allelochemicals.
- Rye exhibits specific biochemical adaptations to interspecific competition.
