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Synthesis and characterization of self-assembling chitosan-based nanoparticles.

Junaid Qureshi1, Furqan Muhamad Iqbal1, Zeeshan Danish2

  • 1Department of Pharmaceutics, Faculty of Pharmacy, Bahauddin Zakariya University, Multan, Pakistan.

Pakistan Journal of Pharmaceutical Sciences
|March 1, 2022
PubMed
Summary

Biodegradable nanovectors were created using modified chitosan (CHT) and polyethylene glycol (PEG). These nanovectors showed promising non-toxicity for potential in-vivo applications, particularly with liver cancer cells.

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Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Polymer Chemistry

Background:

  • Chitosan (CHT) is a biodegradable polymer with potential in drug delivery.
  • Modification of CHT with polyethylene glycol (PEG) can improve its properties.
  • Developing safe and effective nanocarriers is crucial for biomedical applications.

Purpose of the Study:

  • To synthesize and characterize PEGylated chitosan (CHT) nanovectors.
  • To evaluate the physicochemical properties of the developed nanocarriers.
  • To assess the in-vitro toxicity and suitability of nanovectors for potential in-vivo applications.

Main Methods:

  • Chitosan phthaloylation followed by PEGylation with PEG-4000.
  • Nanocarrier formation using the ionic gelation method with sodium tripolyphosphate (NaTPP).
  • Physicochemical characterization including FTIR, SEM, DLS, and zeta potential measurements.
  • In-vitro cytotoxicity assessment using Hep G2 liver cancer cells.

Main Results:

  • Successful synthesis and PEGylation of chitosan confirmed by FTIR.
  • Nanocarriers exhibited particle sizes in the range of 159-170nm.
  • Positive zeta potential (+0.907mV) indicated cationic nanovectors.
  • Cell viability studies demonstrated a concentration-dependent effect, with decreased viability at higher concentrations.

Conclusions:

  • PEGylated chitosan nanovectors were successfully prepared.
  • The nanovectors possess suitable physicochemical properties for potential drug delivery.
  • The study established a non-toxic profile for these nanovectors at specific concentrations, indicating their potential for in-vivo applications.