Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Type III Secretion System plasmid pPHDPT3 of Photobacterium damselae subsp. piscicida is stable in Australian isolates due to conserved non-repetitive genomic architecture.

FEMS microbiology letters·2026
Same author

Evaluating the efficacy of ceftazidime/avibactam plus amikacin combination therapy as a carbapenem-sparing agent against ceftazidime/avibactam-resistant <i>Escherichia coli</i> using a hollow fiber infection model: a proof-of-concept study.

Antimicrobial agents and chemotherapy·2026
Same author

Characterization of a conserved outer-membrane protein in non-typeable <i>Haemophilus influenzae</i> with an unidentified impact on phenotype.

Microbiology spectrum·2026
Same author

NeuO-mediated O-acetylation of uropathogenic <i>Escherichia coli</i> K1 capsule enhances resistance to phage and neutrophil killing.

Journal of bacteriology·2026
Same author

Qualitative insights into vaccine hesitancy among striped catfish (Pangasianodon hypophthalmus) farmers in the Mekong Delta, Vietnam.

Preventive veterinary medicine·2025
Same author

Snapshot Genomic Surveillance Reveals Insights into Antimicrobial Resistance and Lineage Diversity of Uropathogens in Older Adults in Queensland.

Open forum infectious diseases·2025

Related Experiment Video

Updated: Oct 2, 2025

Non-invasive Imaging of the Innate Immune Response in a Zebrafish Larval Model of Streptococcus iniae Infection
11:16

Non-invasive Imaging of the Innate Immune Response in a Zebrafish Larval Model of Streptococcus iniae Infection

Published on: April 21, 2015

10.9K

A high-quality reference genome for the fish pathogen Streptococcus iniae.

Areej S Alsheikh-Hussain1,2, Nouri L Ben Zakour1,2,3, Brian M Forde1,2

  • 1School of Chemistry & Molecular Biosciences, The University of Queensland, Brisbane, Queensland, Australia.

Microbial Genomics
|March 1, 2022
PubMed
Summary

Streptococcus iniae causes significant fish disease and economic losses in aquaculture. This study provides a high-quality genome sequence for S. iniae QMA0248, improving future genomic and evolutionary research.

Keywords:
SMRT sequencinginsertion sequencemisassemblymobile genetic elementsreference-guided assembly

More Related Videos

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
09:44

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

Published on: December 23, 2022

2.5K
Single-Cell Suspension Preparation from Nile Tilapia Intestine for Single-Cell Sequencing
05:34

Single-Cell Suspension Preparation from Nile Tilapia Intestine for Single-Cell Sequencing

Published on: February 10, 2023

1.9K

Related Experiment Videos

Last Updated: Oct 2, 2025

Non-invasive Imaging of the Innate Immune Response in a Zebrafish Larval Model of Streptococcus iniae Infection
11:16

Non-invasive Imaging of the Innate Immune Response in a Zebrafish Larval Model of Streptococcus iniae Infection

Published on: April 21, 2015

10.9K
Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
09:44

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

Published on: December 23, 2022

2.5K
Single-Cell Suspension Preparation from Nile Tilapia Intestine for Single-Cell Sequencing
05:34

Single-Cell Suspension Preparation from Nile Tilapia Intestine for Single-Cell Sequencing

Published on: February 10, 2023

1.9K

Area of Science:

  • Microbiology
  • Genomics
  • Aquaculture

Background:

  • * Streptococcus iniae is a significant pathogen causing fish mortality and economic losses in global aquaculture.
  • * Zoonotic transmission of S. iniae to humans is a potential risk through handling contaminated fish.

Purpose of the Study:

  • * To present the complete genome sequence of Streptococcus iniae strain QMA0248, isolated from farmed barramundi.
  • * To provide a high-quality reference genome for S. iniae, aiding comparative genomic and evolutionary studies.

Main Methods:

  • * Whole-genome sequencing of S. iniae QMA0248 using PacBio and Nanopore technologies.
  • * Comparative and phylogenetic analysis with existing S. iniae genomes.
  • * Long-range PCR and sequence read analysis to investigate rRNA loci and mobile genetic elements.

Main Results:

  • * A 2.12 Mb genome sequence for S. iniae QMA0248 was determined, containing a prophage, genomic island, and 92 insertion sequence (IS) elements, including nine novel types.
  • * Discrepancies were identified in publicly available S. iniae genomes, likely due to misassembly.
  • * Evidence suggests a subpopulation of S. iniae QMA0248 possesses tandem rRNA repeats, which became the prevalent genotype under laboratory conditions, indicating selective pressure.

Conclusions:

  • * The study provides a high-quality, manually curated reference genome for S. iniae QMA0248.
  • * Identified assembly issues in existing S. iniae genomes highlight the need for rigorous genome validation.
  • * The findings offer insights into the genomic diversity and evolutionary dynamics of S. iniae, crucial for aquaculture health management.