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Related Experiment Video

Updated: Oct 1, 2025

Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens
08:49

Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens

Published on: June 6, 2020

14.8K

Identification of cell type specific ACE2 modifiers by CRISPR screening.

Emily J Sherman1, Carmen Mirabelli2, Vi T Tang3,4

  • 1Department of Internal Medicine, Division of Hospital Medicine, University of Michigan, Ann Arbor, Michigan, United States of America.

Plos Pathogens
|March 1, 2022
PubMed
Summary

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Researchers identified key host genes controlling the surface abundance of Angiotensin-Converting Enzyme 2 (ACE2), a crucial receptor for SARS-CoV-2 entry. This discovery offers potential new therapeutic targets for COVID-19 treatment.

Area of Science:

  • Virology
  • Genetics
  • Cell Biology

Background:

  • SARS-CoV-2 uses the ACE2 receptor on host cells for viral entry.
  • ACE2 expression varies significantly, and the underlying molecular mechanisms are not fully understood.

Purpose of the Study:

  • To identify host factors that regulate the surface abundance of ACE2.
  • To understand the cell-type specificity of ACE2 regulatory networks.
  • To uncover potential therapeutic targets for SARS-CoV-2 infection.

Main Methods:

  • High-throughput CRISPR screens were employed to identify genes influencing ACE2 surface levels.
  • Functional validation was performed on specific gene disruptions in liver (HuH7) and lung (Calu-3) cell lines.
  • ACE2 mRNA levels and cellular susceptibility to SARS-CoV-2 infection were assessed.

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Related Experiment Videos

Last Updated: Oct 1, 2025

Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens
08:49

Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens

Published on: June 6, 2020

14.8K
Pooled CRISPR-Based Genetic Screens in Mammalian Cells
09:05

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Published on: September 4, 2019

22.3K
Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes
08:32

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes

Published on: May 23, 2025

539

Main Results:

  • In HuH7 cells, disruption of 35 genes altered ACE2 surface abundance, including transcription factors and epigenetic regulators.
  • SMAD4, EP300, PIAS1, and BAMBI were confirmed to regulate ACE2 mRNA and SARS-CoV-2 susceptibility.
  • Calu-3 cells exhibited a distinct set of ACE2 modifiers, including ACE2 itself, KDM6A, MOGS, GPAA1, and UGP2.

Conclusions:

  • Host factors significantly influence ACE2 surface expression and SARS-CoV-2 entry.
  • ACE2 regulatory networks show cell-type specificity.
  • Identified host factors represent potential therapeutic targets for mitigating SARS-CoV-2 infection.