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[Fibrinolytic activity of Bacillus mesentericus strains].
Mikrobiologiia
|March 1, 1986
Summary
Researchers identified Bacillus mesentericus strains producing potent proteolytic enzymes for thrombolysis. A specific potato broth, peptone, and lactose medium optimized protease synthesis, enabling rapid in vitro dissolution of human blood clots.
Area of Science:
- Microbiology
- Biochemistry
- Enzymology
Background:
- Bacillus mesentericus strains possess proteolytic enzymes.
- Some proteolytic enzymes exhibit thrombolytic properties.
- Optimizing enzyme synthesis is crucial for therapeutic applications.
Purpose of the Study:
- To select Bacillus mesentericus strains with high thrombolytic enzyme activity.
- To investigate the impact of carbon sources on protease synthesis.
- To determine an optimal growth medium for enhanced thrombolytic protease production.
Main Methods:
- Screening of Bacillus mesentericus collection strains for proteolytic and thrombolytic activity.
- Cultivation of selected strains in media with varying carbon sources.
- In vitro assessment of the thrombolytic activity of the resulting cultural broth.
Main Results:
- Two Bacillus mesentericus strains with significant thrombolytic enzyme activity were identified.
- A specific growth medium (10% potato broth, 0.5% peptone, 0.5% lactose) was found to maximize protease synthesis.
- The optimized cultural broth demonstrated rapid in vitro dissolution of human blood clots within 2.5 to 3 hours.
Conclusions:
- Bacillus mesentericus is a viable source of thrombolytic enzymes.
- Medium composition significantly influences protease production and thrombolytic efficacy.
- The developed medium offers a promising approach for producing effective thrombolytic agents.