Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Converging Effects of Different Categories of Antidepressants on the Brain: A Systematic Meta-Analysis of Public Transcriptional Profiling Data From the Hippocampus and Cortex.

Journal of neurochemistry·2026
Same author

Virtual Reality Education for Hospitalized Pediatric Patients Improves Intrinsic Motivation: A Prospective, Randomized Crossover Study.

Clinical pediatrics·2026
Same author

The Converging Effects of Different Categories of Antidepressants on the Brain: A Systematic Meta-Analysis of Public Transcriptional Profiling Data from the Hippocampus and Cortex.

bioRxiv : the preprint server for biology·2026
Same author

Brain-Immune Crosstalk in Mental Illness: The Path to Immunopsychiatry.

Biological psychiatry·2026
Same author

Impact of CYP and ABCB1 Polymorphisms on Bortezomib-Induced Adverse Events in Multiple Myeloma.

Biomedicines·2026
Same author

Lung adventitial fibroblasts support type 2 Tregs to shape the response to influenza infection.

bioRxiv : the preprint server for biology·2026
Same journal

A human-specific genetic modifier reconfigures large-scale cortical network dynamics underlying behavioral performance.

bioRxiv : the preprint server for biology·2026
Same journal

<i>Staphylococcus aureus</i> uses a eukaryotic-like uridyltransferase to make UDP-GlcNAc for cell wall synthesis.

bioRxiv : the preprint server for biology·2026
Same journal

Dynamic redistribution of eIF4F controls cap-dependent translation initiation.

bioRxiv : the preprint server for biology·2026
Same journal

When does additional information improve accuracy of RNA secondary structure prediction?

bioRxiv : the preprint server for biology·2026
Same journal

Normative brain-state trajectories reveal deviation from healthy aging in Alzheimer's disease.

bioRxiv : the preprint server for biology·2026
Same journal

Noradrenergic infraslow rhythm during sleep is the critical link between heart-rate dynamics and memory consolidation.

bioRxiv : the preprint server for biology·2026
See all related articles

Related Experiment Video

Updated: Oct 1, 2025

Isolation of Cortical Microglia with Preserved Immunophenotype and Functionality From Murine Neonates
09:12

Isolation of Cortical Microglia with Preserved Immunophenotype and Functionality From Murine Neonates

Published on: January 30, 2014

16.2K

Type I interferon responsive microglia shape cortical development and behavior.

Caroline C Escoubas1, Leah C Dorman1,2, Phi T Nguyen1,3

  • 1Department of Psychiatry and Behavioral Sciences/ Weill Institute for Neurosciences, University of California, San Francisco, San Francisco, CA.

Biorxiv : the Preprint Server for Biology
|March 2, 2022
PubMed
Summary
This summary is machine-generated.

Microglia use Type I interferon (IFN-I) signaling to engulf neurons during brain development. This pathway is crucial for clearing damaged neurons and normal circuit formation.

More Related Videos

Immunofluorescence Staining Using IBA1 and TMEM119 for Microglial Density, Morphology and Peripheral Myeloid Cell Infiltration Analysis in Mouse Brain
10:40

Immunofluorescence Staining Using IBA1 and TMEM119 for Microglial Density, Morphology and Peripheral Myeloid Cell Infiltration Analysis in Mouse Brain

Published on: October 27, 2019

33.2K
Characterization and Isolation of Mouse Primary Microglia by Density Gradient Centrifugation
10:21

Characterization and Isolation of Mouse Primary Microglia by Density Gradient Centrifugation

Published on: February 16, 2018

19.6K

Related Experiment Videos

Last Updated: Oct 1, 2025

Isolation of Cortical Microglia with Preserved Immunophenotype and Functionality From Murine Neonates
09:12

Isolation of Cortical Microglia with Preserved Immunophenotype and Functionality From Murine Neonates

Published on: January 30, 2014

16.2K
Immunofluorescence Staining Using IBA1 and TMEM119 for Microglial Density, Morphology and Peripheral Myeloid Cell Infiltration Analysis in Mouse Brain
10:40

Immunofluorescence Staining Using IBA1 and TMEM119 for Microglial Density, Morphology and Peripheral Myeloid Cell Infiltration Analysis in Mouse Brain

Published on: October 27, 2019

33.2K
Characterization and Isolation of Mouse Primary Microglia by Density Gradient Centrifugation
10:21

Characterization and Isolation of Mouse Primary Microglia by Density Gradient Centrifugation

Published on: February 16, 2018

19.6K

Area of Science:

  • Neuroscience
  • Immunology
  • Developmental Biology

Background:

  • Microglia, the brain's immune cells, perform various phagocytic functions, including engulfing neurons.
  • The specific molecular mechanisms governing distinct microglial phagocytic states, particularly neuronal engulfment, remain unclear.

Approach:

  • Transcriptomic analysis identified a specific subset of Type I interferon (IFN-I) responsive microglia in the developing somatosensory cortex.
  • Investigated the role of IFN-I signaling in microglial phagocytosis of neurons using conditional gene deletion and exogenous IFN-I administration in mice.

Key Points:

  • IFN-I responsive microglia exhibit heightened phagocytic activity, actively engulfing whole neurons in the developing brain.
  • Deletion of IFN-I signaling in microglia impairs neuronal engulfment, leading to accumulation of DNA-damaged neurons.
  • Exogenous IFN-I promotes microglial neuronal engulfment and prevents damaged neuron buildup.

Conclusions:

  • This study defines a novel molecular mechanism involving IFN-I signaling that regulates microglial engulfment of neurons during a critical developmental window.
  • Reveals essential homeostatic functions of the canonical antiviral IFN-I pathway in mammalian brain development and neural circuit refinement.