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Related Experiment Video

Updated: Oct 1, 2025

Somatic Genome-Engineered Mouse Models Using In Vivo Microinjection and Electroporation

Somatic Genome-Engineered Mouse Models Using In Vivo Microinjection and Electroporation

Published on: May 5, 2023

Mouse Model for Optogenetic Genome Engineering.

Tomoka Takao¹, Daisuke Yamada¹, Takeshi Takarada¹

¹Department of Regenerative Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences.

Acta Medica Okayama

|March 3, 2022

Summary

This summary is machine-generated.

Optogenetics uses light to control biological processes. Researchers developed the Magnet System for precise control and created new mice for advanced optogenetic studies.

Keywords:

Cre recombinase optogenetics

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Published on: April 2, 2014

Area of Science:

Neuroscience
Molecular Biology
Biotechnology

Background:

Optogenetics is a powerful tool for manipulating biological phenomena using light, gaining significant traction in neuroscience research.
The Magnet System, a photo-inducible protein dimerization system, offers high accuracy in controlling intracellular biomolecule behavior with light.

Purpose of the Study:

To review the historical development of optogenetics.
To introduce the latest advancements in Magnet System technology.
To present newly developed photoactivatable Cre knock-in mice for precise biological control.

Main Methods:

Review of optogenetics history and Magnet System technology.
Integration of Magnet System with genome-editing (CRISPR/Cas9) and DNA recombination (Cre-loxP) technologies.
Development of photoactivatable Cre knock-in mice.

Main Results:

The Magnet System enables accurate, light-induced control of intracellular biomolecules.
Combination with CRISPR/Cas9 and Cre-loxP systems enhances photoactivation capabilities.
Successfully generated photoactivatable Cre knock-in mice exhibiting temporal, spatial, and cell-specific accuracy.

Conclusions:

Optogenetics, particularly with the Magnet System, provides sophisticated tools for biological research.
The developed photoactivatable Cre knock-in mice represent a significant advancement for precise optogenetic manipulation.
These tools offer enhanced control for studying complex biological phenomena in neuroscience.