Chick Embryo Ex Vivo Aortic Sprouting Assays for Cardiovascular Research

Angiogenesis is the formation of new blood vesicles and is controlled by a dynamic cascade of molecular and cellular activities. The whole procedure can be replicated in vitro under chemically specified conditions by cultivating chick aortic explants in biomatrices. In this technique, angiogenesis is powered by endogenous molecules that the aorta releases to promote its outgrowth. In an ordered series of morphogenetic events, sprouting endothelial cells are strongly associated with macrophages, fibroblasts, and pericytes, recapitulating all the phases of the angiogenic process. The structural, morphologic, and molecular properties of the angiogenic process can be studied and the effectiveness of pro/antiangiogenic drugs can also be evaluated using this aortic culture. We describe in this chapter the basic procedure currently used in our laboratory to measure the angiogenic properties for cardiovascular research.