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Evaluation of the Storage Stability of Extracellular Vesicles
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Extracellular Vesicles in Atherosclerosis Research.

Jessica O Williams1, Cass Whelan1, Jamie Nash1

  • 1Cardiff School of Sport and Health Sciences, Cardiff Metropolitan University, Cardiff, UK.

Methods in Molecular Biology (Clifton, N.J.)
|March 3, 2022
PubMed
Summary
This summary is machine-generated.

This chapter details methods for using extracellular vesicles (EV) in model systems to study their role in atherosclerosis. These techniques can be applied to investigate EV involvement in plaque development and thrombus formation.

Keywords:
AtherosclerosisExtracellular vesiclesMonocyte migrationStatic adhesionThrombus formation

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Area of Science:

  • Cardiovascular Research
  • Cell Biology
  • Biotechnology

Background:

  • Atherosclerosis is a complex disease involving plaque formation.
  • Extracellular vesicles (EV) are implicated in various pathological processes.
  • Understanding the role of EV in atherosclerosis requires robust experimental models.

Purpose of the Study:

  • To provide methodologies for incorporating extracellular vesicles (EV) into model systems.
  • To investigate the specific roles of EV in the initiation and progression of atherosclerotic plaques.
  • To offer versatile techniques applicable across different cell types and isolation conditions.

Main Methods:

  • Incorporation of extracellular vesicles (EV) into established atherosclerosis model systems.
  • Assays to evaluate EV impact on coagulation and thrombus formation.
  • Assessment of EV effects on monocytic migration and endothelial cell adhesion.

Main Results:

  • The described methodologies enable the investigation of EV functions in atherosclerosis models.
  • Specific EV-mediated processes such as coagulation, thrombus formation, and cell migration can be studied.
  • The techniques are adaptable for EVs derived from various sources and experimental contexts.

Conclusions:

  • This chapter presents adaptable methodologies for studying extracellular vesicles in atherosclerosis.
  • The described techniques facilitate research into EV's contribution to atherosclerotic plaque development.
  • These methods support the exploration of EV roles in key pathological events relevant to cardiovascular disease.