Related Experiment Videos
Revised classification of the DLA loci by serological studies
Tissue Antigens
|May 1, 1986
Summary
Canine leukocyte antigen (DLA)-B antigens are not associated with beta 2-microglobulin, suggesting they may be canine class II gene products. DLA-A antigens, however, are associated with beta 2-microglobulin.
Area of Science:
- Immunogenetics
- Veterinary Immunology
- Molecular Biology
Background:
- The canine leukocyte antigen (DLA) system is crucial for immune responses and transplantation in dogs.
- Beta 2-microglobulin is a component of major histocompatibility complex (MHC) class I molecules in many species.
Purpose of the Study:
- To investigate the association of DLA class I molecules with beta 2-microglobulin.
- To differentiate DLA-A, DLA-B, and DLA-C antigen associations with beta 2-microglobulin.
Main Methods:
- Peripheral blood mononuclear cells from DLA-typed dogs were treated to aggregate DLA class I molecules (lysostrip).
- Cells were subsequently tested for complement-dependent lysis using DLA-A, DLA-B, and DLA-C specific antisera.
Main Results:
- Lysostrip treatment blocked complement-dependent lysis mediated by DLA-A specific antisera.
- Reactivity with DLA-B specific antisera was not blocked; complete lympholysis was observed.
- DLA-C antisera showed variable reactions, preventing definitive assignment.
Conclusions:
- DLA-B antigens are not associated with beta 2-microglobulin and are potential candidates for canine class II gene products.
- DLA-A antigens are associated with beta 2-microglobulin, consistent with their role as class I molecules.