[A solid-phase immunoenzyme method of the quantitative determination of insulin antibodies]

A test system for quantitative estimation of antibodies towards insulin was developed using the solid phase immunoenzymatic procedure. The test system was calibrated using affinity antibodies towards insulin, obtained after immunosorption of blood plasma of insulin-resistant patient with diabetes. The normal content of autoantibodies, estimated by means of the procedure in human blood serum, constituted 35.2 X 10(-3) +/- 12.4 X 10(-3) g/l. In the patients with diabetes increased content of autoantibodies towards insulin was usually determined--70 X 10(-3)-180 X 10(-3) g/l.