Vasa identifies germ cells in embryos and gonads of Oryzias celebensis

Vasa is the most studied germ cell marker that is indispensable for germ cell development in teleost fishes. Here, a vasa full-length cDNA from Oryzias celebensis was isolated. Analysis of gene expression by reversed transcription polymerase chain reaction and in situ hybridization showed the vasa transcript was maternally inherited and specifically expressed in germ cells during embryogenesis and in adult gonads. During embryogenesis, vasa mRNA was widely distributed in the embryos until the somitogenesis stage and then specifically expressed in primordial germ cells (PGCs). In the testis, vasa expression was highest in spermatogonia and gradually decreased during spermatogenesis. In ovary, vasa expression was present predominantly in immature oocytes and persisted throughout oogenesis. Constructs containing green or red fluorescence proteins and vasa 3' UTR or dnd 3' UTR, confirmed stable vasa expression in the PGCs of O. celebensis and co-expression of the two genes. In summary, the conservation of vasa expression in embryonic and adult germ cells of both sexes compared to other vertebrates suggests its function is also widely conserved.