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Related Experiment Videos

A means for orienting flat cells in flow systems.

R T Stovel, R G Sweet, L A Herzenberg

    Biophysical Journal
    |July 1, 1978
    PubMed
    Summary
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    This study introduces a novel flow geometry to orient flattened cells, improving cell analysis accuracy. The new system ensures consistent cell presentation to detectors, reducing measurement variability in flow cytometry.

    Area of Science:

    • Biotechnology
    • Analytical Chemistry
    • Cell Biology

    Background:

    • Flattened cells (e.g., red blood cells, epithelial cells) pose orientation challenges in flow cytometry.
    • Inconsistent cell orientation leads to significant variability in fluorescence and light-scattering measurements.
    • Current flow systems lack control over the orientation of non-spherical cells.

    Purpose of the Study:

    • To develop and describe a flow geometry that consistently orients flattened cells in coaxial flow systems.
    • To enable identical cell aspect presentation to detectors for improved measurement accuracy.
    • To address the limitations of current cell analysis and sorting machines with respect to cell orientation.

    Main Methods:

    • A coaxial flow system was employed.

    Related Experiment Videos

  • A wedge-shaped exit was integrated into the sample injection tube.
  • Low sample flow rates were utilized to achieve orientation.
  • Fixed chicken erythrocytes were used to demonstrate the orientation effect.

    • Main Results:

    • The described flow geometry successfully orients flattened cells.
    • Consistent cell aspect presentation to observation devices was achieved.
    • Variability in light-scattering measurements was reduced, as shown with chicken erythrocytes.

    Conclusions:

    • A simple modification to flow geometry can effectively orient flattened cells.
    • This orientation method enhances the reliability of fluorescence-activated cell analysis and sorting.
    • The findings have implications for improving the precision of various cell analysis techniques.