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Updated: Oct 1, 2025

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LiverClear: A versatile protocol for mouse liver tissue clearing.

Laura M Molina1,2, Yekaterina Krutsenko2, Nathaniel E C Jenkins3

  • 1Medical Scientist Training Program, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA.

STAR Protocols
|March 4, 2022
PubMed
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This summary is machine-generated.

A new protocol, LiverClear, effectively clears mouse and human liver tissue for 3D imaging. This method enables visualization of intricate structures like bile ducts and hepatocyte canaliculi in both healthy and diseased samples.

Area of Science:

  • Anatomical Pathology
  • Biotechnology
  • Microscopy

Background:

  • Existing tissue clearing protocols often fail to adequately clear dense liver tissue.
  • The liver's complex 3D architecture presents significant challenges for traditional imaging techniques.

Purpose of the Study:

  • To develop a versatile and effective tissue clearing protocol specifically for mouse and human liver samples.
  • To enable high-resolution 3D imaging of intact liver structures.

Main Methods:

  • A novel protocol, LiverClear, was developed by combining and optimizing strategies for delipidation, decolorization, and refractive index matching.
  • The protocol integrates immunofluorescence staining for detailed structural visualization.
  • Adapted for both healthy and diseased liver tissues from mice and humans.
Keywords:
Developmental biologyMicroscopy

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Main Results:

  • LiverClear successfully cleared mouse liver tissue, allowing for 3D visualization of intact bile ducts and hepatocyte canaliculi.
  • The protocol demonstrated efficacy in clearing both healthy and diseased liver samples.
  • Adaptation for human liver clearing was also successful, broadening its applicability.

Conclusions:

  • LiverClear provides a robust and flexible solution for clearing liver tissue, overcoming limitations of previous methods.
  • This protocol facilitates advanced 3D imaging, offering new insights into liver structure and disease.