LiverClear: A versatile protocol for mouse liver tissue clearing
Related Experiment Video
Updated: Oct 1, 2025
Imaging and Quantification of Intact Neuronal Dendrites via CLARITY Tissue Clearing
Published on: April 20, 2021
Laura M Molina1,2, Yekaterina Krutsenko2, Nathaniel E C Jenkins3
1Medical Scientist Training Program, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA.
Abstract:
Although there are numerous tissue clearing protocols, most are inadequate for clearing liver tissue. Here we present a flexible protocol for mouse liver tissue; we combine strategies from several previously published protocols for delipidation, decolorization, staining, and refractive index matching. LiverClear is sufficiently versatile to allow clearing of healthy and diseased mouse liver followed by immunofluorescence staining and imaging to visualize intact 3D structures such as bile ducts and hepatocyte canaliculi. We also adapted this protocol for clearing human livers. For complete details on the use and execution of this protocol, please refer to Molina et al. (2021).
