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Dexamethasone-loaded keratin films for ocular surface reconstruction
Rebekka Schwab1, Stephan Reichl2
1Institut für Pharmazeutische Technologie, Technische Universität Braunschweig, Braunschweig, Germany.
Journal of Materials Science. Materials in Medicine
|March 4, 2022
Summary
Keratin films (KFs) loaded with dexamethasone (DEX) offer a promising alternative for ocular surface reconstruction. These drug-eluting films enhance anti-inflammatory treatment compliance and maintain beneficial film characteristics.
Area of Science:
- Biomaterials Science
- Ophthalmology
- Regenerative Medicine
Background:
- Amniotic membrane (AM) is a common material for ocular surface reconstruction but has limitations.
- Keratin films (KFs) derived from human hair show potential as AM alternatives due to favorable characteristics and biocompatibility.
- Post-surgical inflammation management with dexamethasone (DEX) eye drops poses compliance challenges.
Purpose of the Study:
- To incorporate dexamethasone (DEX) into keratin films (KFs) for sustained local delivery.
- To evaluate the impact of DEX incorporation on KF properties and biocompatibility.
- To identify optimal methods for DEX integration into KFs for ocular surface reconstruction.
Main Methods:
- DEX-loaded KFs were prepared using suspension or solubilizing agent techniques.
- Films were analyzed for appearance, loading capacity, transparency, mechanical properties, swelling, and in vitro DEX release.
- In vitro biocompatibility was assessed using corneal epithelial cells (viability, seeding, growth).
Main Results:
- DEX incorporation affected KF transparency and biomechanics, but properties remained comparable to AM.
- The suspension technique yielded superior chemical stability and prolonged DEX release.
- DEX-loaded KFs did not impair cell seeding efficiency, and moderate DEX loads supported satisfactory cell growth.
Conclusions:
- DEX-loaded KFs are a viable alternative material for ocular surface reconstruction.
- These films offer integrated anti-inflammatory activity, potentially improving patient compliance.
- The suspension method is optimal for creating stable, drug-eluting KFs for ophthalmic applications.
