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Updated: Oct 1, 2025

Structure-function Studies in Mouse Embryonic Stem Cells Using Recombinase-mediated Cassette Exchange
Published on: April 27, 2017
Biophysical and molecular mechanisms of ESCRT functions, and their implications for disease
Simona Maria Migliano1, Eva Maria Wenzel1, Harald Stenmark1
1Centre for Cancer Cell Reprogramming, Institute of Clinical Medicine, Faculty of Medicine, University of Oslo, Montebello, N-0379 Oslo, Norway; Department of Molecular Cell Biology, Institute for Cancer Research, Oslo University Hospital, Montebello, N-0379 Oslo, Norway.
Abstract:
The endosomal sorting complex required for transport (ESCRT) machinery evolved early in evolution to sculpt and cut cellular membranes. Consisting of three subcomplexes termed ESCRT-I, -II and -III, this machinery is recruited to various cellular locations to perform key steps in essential processes such as protein degradation, cell division, and membrane sealing. Here we review recent discoveries that have shed light on biophysical and molecular mechanisms of ESCRTs in endolysosomal protein degradation and nuclear envelope sealing, and we discuss how dysfunctional ESCRTs can lead to diseases such as cancer and neurodegenerative disorders.
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