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Complete Genome Sequence of Stenotrophomonas maltophilia Siphophage Summit

Alexandra Bishop1, Xing-Han Zhang1, James Clark2

  • 1Department of Biochemistry and Biophysics, Texas A&M University, College Station, Texas, USA.

Microbiology Resource Announcements
|March 7, 2022
PubMed

Insights

Researchers sequenced the genome of the Stenotrophomonas maltophilia siphophage, Summit. This analysis identified 148 protein-coding genes and 5 tRNAs, including a potential suppressor tRNA, offering insights into phage biology.

Area of Science:

  • Microbiology
  • Genomics
  • Bacteriology

Background:

  • Stenotrophomonas maltophilia is an opportunistic pathogen known for its multidrug resistance.
  • Bacteriophages (phages) are viruses that infect bacteria and can be specific to certain bacterial hosts.

Purpose of the Study:

  • To report the complete genome sequence of a siphophage, named Summit, that infects Stenotrophomonas maltophilia.
  • To characterize the genetic content of the Summit phage, including protein-coding genes and tRNAs.

Main Methods:

  • Whole-genome sequencing of the S. maltophilia siphophage Summit.
  • Bioinformatic analysis to identify protein-coding genes and transfer RNA (tRNA) genes within the phage genome.

Main Results:

  • The complete genome of the Summit siphophage was determined to be 95,728 base pairs.
  • A total of 148 protein-coding genes and 5 tRNAs were identified.
  • One predicted suppressor tRNA was found, though its specific target genes remain unidentified.

Conclusions:

  • The genome of the S. maltophilia siphophage Summit has been fully elucidated.
  • The identified genetic components provide a foundation for understanding the biology and potential applications of this phage.

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