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Long non-coding RNA long intergenic non-coding 00641 mediates cell progression with stimulating cisplatin-resistance

JinShan Tang1,2, ZiQiang Zhu3,4, Suwei Dong3,4

  • 1Department of Orthopedic, The Second People's Hospital of Huai'an, Huai'an City, Jiangsu Province, China.

Bioengineered
|March 10, 2022
PubMed

Insights

Long non-coding RNA LINC00641 promotes cisplatin resistance in osteosarcoma by regulating the miR-320d/MCL1 pathway. Reducing LINC00641 enhances chemotherapy sensitivity in osteosarcoma.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Cisplatin (DDP) is a key chemotherapy drug for osteosarcoma (OS), but drug resistance limits its efficacy.
  • Long non-coding RNAs (lncRNAs) are implicated in OS development, yet the role of LINC00641 in DDP resistance is unclear.

Purpose of the Study:

  • To investigate the function and mechanism of lncRNA LINC00641 in DDP resistance in osteosarcoma.

Main Methods:

  • Analysis of LINC00641, miR-320d, and MCL1 expression in clinical OS tissues and cell lines.
  • In vitro experiments assessing cell progression and drug resistance after LINC00641 manipulation.
  • Verification of molecular interactions using bioinformatics and experimental assays.
  • In vivo xenograft models to evaluate LINC00641's role in DDP resistance.

Main Results:

  • LINC00641 expression was significantly upregulated in OS tissues and cells, particularly in DDP-resistant samples.
  • Knockdown of LINC00641 reduced OS cell progression and DDP resistance in vitro and in vivo.
  • LINC00641 directly binds to miR-320d, modulating MCL1 expression and promoting DDP resistance via the miR-320d/MCL1 axis.

Conclusions:

  • LINC00641 acts as a crucial oncogenic lncRNA that enhances DDP resistance in osteosarcoma.
  • Targeting the LINC00641/miR-320d/MCL1 pathway may represent a therapeutic strategy to overcome DDP resistance in OS.
Keywords:
Long intergenic non-coding 00641apoptosiscisplatin-resistanceosteosarcomaproliferation

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