Related Experiment Video

Updated: Sep 30, 2025

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

7.7K

Long non-coding RNA TTTY15 silencing inhibits gastric cancer progression by sponging microRNA-98-5p to down-regulate

Xigang Wen1, Wenling Han2, Chao Liu1

  • 1Department of Gastrointestinal Surgery, The Third People's Hospital of Hubei Province, Wuhan, China.

Bioengineered
|March 10, 2022
PubMed

Insights

Long non-coding RNA TTTY15 promotes gastric cancer by sponging miR-98-5p, leading to increased cyclin D2 expression. This study identifies TTTY15 as a potential therapeutic target for gastric cancer treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Gastric cancer (GC) is a leading cause of cancer-related mortality globally.
  • Early detection of gastric cancer remains challenging, leading to poor patient outcomes.
  • Identifying novel therapeutic targets is crucial for improving gastric cancer treatment strategies.

Purpose of the Study:

  • To investigate the role of long non-coding RNA (lncRNA) TTTY15 in gastric cancer.
  • To elucidate the molecular mechanism underlying TTTY15's function in gastric cancer progression.
  • To evaluate TTTY15 as a potential diagnostic biomarker and therapeutic target for gastric cancer.

Main Methods:

  • Quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blot assays were used to assess the expression of TTTY15, miR-98-5p, and cyclin D2 (CCND2).
  • Bioinformatics, dual-luciferase assays, and RNA pull-down assays were employed to verify the interactions between TTTY15, miR-98-5p, and CCND2.
  • Cell proliferation was assessed using MTT assays, and apoptosis was measured by flow cytometry and caspase-3 assays.

Main Results:

  • TTTY15 and CCND2 expression levels were significantly upregulated in gastric cancer tissues and cell lines, while miR-98-5p expression was downregulated.
  • Knockdown of TTTY15 inhibited gastric cancer cell proliferation and promoted apoptosis.
  • TTTY15 functions by sponging miR-98-5p, which in turn suppresses CCND2 expression, indicating a tumor-suppressive role for miR-98-5p.

Conclusions:

  • lncRNA TTTY15 acts as an oncogene in gastric cancer by regulating the miR-98-5p/CCND2 axis.
  • TTTY15 may serve as a novel therapeutic target for gastric cancer.
  • Further research into TTTY15's role could lead to improved diagnostic and therapeutic strategies for gastric cancer.
Keywords:
CCND2LncRNA TTTY15gastric cancermiR-98-5p

More Related Videos

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library08:40

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

17.7K

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
22.0K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
9.0K
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.3K
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
17.1K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.7K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
3.9K