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453
Wound-Microenvironment Engineering through Advanced-Dressing Bioprinting
Cristina Del Amo1, Xabier Fernández-San Argimiro2, María Cascajo-Castresana2
1Regenerative Therapies, Bioprinting Laboratory, Biocruces Bizkaia Health Research Institute, Cruces University Hospital, 48903 Barakaldo, Spain.
International Journal of Molecular Sciences
|March 10, 2022
Summary
This study engineered advanced wound dressings using bioprinting techniques. These novel dressings promote cell growth and activate wound healing mechanisms, offering a new strategy for chronic wound management.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Regenerative Medicine
Background:
- Chronic wounds pose a significant economic burden on healthcare systems.
- Engineering the cellular microenvironment is crucial for promoting wound healing.
- Advanced wound dressings are needed to accelerate healing in patients with comorbidities.
Purpose of the Study:
- To develop and characterize novel composite bioinks for advanced wound dressings.
- To investigate the potential of engineered microenvironments in activating cellular wound-healing mechanisms.
- To assess the impact of bioink composition on cell viability, proliferation, and paracrine signaling.
Main Methods:
- Extrusion bioprinting of composite bioinks formulated from adipose-derived decellularized extracellular matrix, plasma, and human dermal fibroblasts.
- Rheological and microstructural analysis of hydrogel properties.
- Assessment of embedded fibroblast viability, proliferation, and extracellular matrix protein expression (collagens, fibronectin).
- ELISA and multiplex protein array analyses to evaluate paracrine factor release and bioinformatic analysis.
- Stimulation of human dermal cells with conditioned media from the engineered dressings.
Main Results:
- The composite hydrogels demonstrated favorable rheological properties supporting cell viability and proliferation.
- Embedded fibroblasts maintained expression of key extracellular matrix proteins.
- Paracrine signaling associated with wound healing, including modulation of inflammation and angiogenesis, was identified.
- Dressing modalities with varying platelet concentrations influenced the release of key cytokines (IL-8, MCP-1, VEGF, HGF).
- Conditioned media promoted human dermal cell proliferation.
Conclusions:
- Engineered microenvironments using bioprinted composite dressings can enhance wound healing.
- The developed bioinks support cell function and promote pro-healing paracrine signaling.
- This approach offers a promising strategy for developing advanced therapies for chronic wounds.
