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Updated: Sep 30, 2025

Imaging Calcium in Drosophila at Egg Activation
Published on: August 6, 2016
Artificial Egg Activation Using Calcium Ionophore
Ahmed Attia1, Cara Nicholson1, Sarah J Martins da Silva1
1Reproductive Medicine Research Group, School of Medicine, Ninewells Hospital and Medical School, University of Dundee, Dundee, United Kingdom.
Abstract:
Artificial oocyte activation, most commonly using calcium ionophore, is a treatment add-on utilized to avoid recurrence of abnormally low or total failed fertilization following in vitro fertilization/intracytoplasmic sperm injection. It aims to modify defective physiological processes, specifically calcium-mediated cell signaling that are critical to events required for fertilization. Routine application of artificial oocyte activation is neither required nor recommended; however, it represents an invaluable intervention for a subgroup of patients affected by sperm-related oocyte activation deficiency.
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