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OMIP 083: A 21-marker 18-color flow cytometry panel for in-depth phenotyping of human peripheral monocytes

Kathryn E Hally1,2,3, Laura Ferrer-Font4, Katherine R Pilkington5

  • 1Department of Surgery and Anaesthesia, The University of Otago, Wellington.

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
|March 11, 2022
PubMed
Abstract

No abstract available in PubMed .

Keywords:
OMIPPBMCsfull spectrum flow cytometryhuman immunophenotypinginnate immunitymonocytes

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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