A Near-Infrared Probe for Specific Imaging of Lipid Droplets in Living Cells

Lipid droplets (LDs) are involved in various physiological processes and associated with cancer development, and are regarded as a potential tumor marker for cancer diagnosis. Monitoring LDs is of prior importance to understand their involvement in biological mechanisms and the early detection of cancers. Highly sensitive and specific noninvasive fluorescent probes are particularly desirable for imaging LDs and cancer diagnosis. Herein, according to the high-viscosity and low-polarity microenvironment in LDs, we developed four easily prepared LDs-specific probes based on noncharged merocyanines. Among them, LD-1 absorbs and emits in the near-infrared (NIR) region with a large Stokes shift. Importantly, LD-1 displayed high sensitivity to high viscosity and low polarity, which allowed it to show high LDs-targeting ability. In cell imaging, LD-1 successfully probed the changes in LDs in the presence of oleic acid or during ferroptosis and was used to distinguish cancer cells from normal cells.