Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Oogenesis02:07

Oogenesis

64.6K
In human women, oogenesis produces one mature egg cell or ovum for every precursor cell that enters meiosis. This process differs in two unique ways from the equivalent procedure of spermatogenesis in males. First, meiotic divisions during oogenesis are asymmetric, meaning that a large oocyte (containing most of the cytoplasm) and minor polar body are produced as a result of meiosis I, and again following meiosis II. Since only oocytes will go on to form embryos if fertilized, this unequal...
64.6K
Menopause01:28

Menopause

708
Menopause, a natural biological process marking the end of a woman's fertility, typically occurs between the fifth and sixth decade of life. This phase is characterized by the exhaustion of the ovarian follicle pool, leading to less responsive ovaries despite the high levels of Follicle Stimulating Hormone (FSH) and Luteinizing Hormone (LH). The consequential decrease in estrogen production results in symptoms like hot flashes, heavy sweating, headaches, hair loss, muscle pains, vaginal...
708
Hormonal Control of the Ovarian Cycle01:30

Hormonal Control of the Ovarian Cycle

3.9K
The ovarian cycle is meticulously regulated by the hypothalamic-pituitary-gonadal axis. This cycle orchestrates the release of a mature oocyte, essential for reproduction.
Before puberty, the hypothalamus releases GnRH in a low frequency, low amplitude pulsatile manner. This along with the immature hypothalamic-pituitary-gonadal axis activity, results in low estrogen levels and the absence of a fully functional ovarian cycle.  At puberty, GnRH secretion increases in both frequency and...
3.9K
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

5.0K
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
5.0K
Electron Transport Chain: Complex I and II01:46

Electron Transport Chain: Complex I and II

15.2K
The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...
15.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

COX-2 inhibition improves immune system homeostasis and decreases liver damage in septic rats.

The Journal of surgical research·2009
Same author

Mass spectral characterization of organophosphate-labeled, tyrosine-containing peptides: characteristic mass fragments and a new binding motif for organophosphates.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences·2009
Same author

3D-SURFER: software for high-throughput protein surface comparison and analysis.

Bioinformatics (Oxford, England)·2009
Same author

Total arch replacement with stented elephant trunk technique: a proposed treatment for complicated Stanford type B aortic dissection.

Journal of cardiac surgery·2009
Same author

Top-emitting white organic light-emitting devices with a one-dimensional metallic-dielectric photonic crystal anode.

Optics letters·2009
Same author

[Detection of tick and tick-borne pathogen in some ports of Inner Mongolia].

Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi·2009
Same journal

Short-term responsiveness of DNA methylation-based aging biomarkers to a multimodal intervention comprising exercise and dietary guidance involving daily consumption of yogurt containing <i>Bifidobacterium longum</i> BB536: an exploratory randomized controlled trial.

Aging·2026
Same journal

Hormonal dimorphism in sarcopenia disease.

Aging·2026
Same journal

The multifaceted inducers of cellular senescence.

Aging·2026
Same journal

Life expectancy and causes of death in classical laminopathic progeroid syndromes: systematic review with individual-patient data synthesis.

Aging·2026
Same journal

Age-related dysfunctions of the neuroendocrine axes in nonhuman primates with depression-like and anxious behavior.

Aging·2026
Same journal

The love and hate relationship between cellular senescence and stemness.

Aging·2026
See all related articles

Related Experiment Video

Updated: Sep 30, 2025

Z-Scores for Assessing Ovarian Reserve in Young Patients Undergoing Fertility Preservation
05:42

Z-Scores for Assessing Ovarian Reserve in Young Patients Undergoing Fertility Preservation

Published on: October 25, 2024

1.3K

Decrease in ovarian reserve through the inhibition of SIRT1-mediated oxidative phosphorylation.

Lu Guo1,2, Xiaocheng Liu1,2, Hua Chen1,2

  • 1Department of Gynecology, Obstetrics and Gynecology Hospital of Fudan University, Shanghai 200011, China.

Aging
|March 11, 2022
PubMed
Summary
This summary is machine-generated.

Oxidative stress induces premature ovarian insufficiency (POI) by inhibiting SIRT1 and mitochondrial function, leading to follicular apoptosis. Restoring SIRT1 activity with resveratrol can improve ovarian reserve and mitochondrial oxidative phosphorylation.

Keywords:
3-nitropropionic acidmouse modelovarian reserveoxidative stresspremature ovarian insufficiency

More Related Videos

Whole Ovary Immunofluorescence, Clearing, and Multiphoton Microscopy for Quantitative 3D Analysis of the Developing Ovarian Reserve in Mouse
12:36

Whole Ovary Immunofluorescence, Clearing, and Multiphoton Microscopy for Quantitative 3D Analysis of the Developing Ovarian Reserve in Mouse

Published on: September 3, 2021

4.9K
Orthotopic Ovarian Transplantation Procedures to Investigate the Life- and Health-span Influence of Ovarian Senescence in Female Mice
06:49

Orthotopic Ovarian Transplantation Procedures to Investigate the Life- and Health-span Influence of Ovarian Senescence in Female Mice

Published on: February 12, 2018

11.1K

Related Experiment Videos

Last Updated: Sep 30, 2025

Z-Scores for Assessing Ovarian Reserve in Young Patients Undergoing Fertility Preservation
05:42

Z-Scores for Assessing Ovarian Reserve in Young Patients Undergoing Fertility Preservation

Published on: October 25, 2024

1.3K
Whole Ovary Immunofluorescence, Clearing, and Multiphoton Microscopy for Quantitative 3D Analysis of the Developing Ovarian Reserve in Mouse
12:36

Whole Ovary Immunofluorescence, Clearing, and Multiphoton Microscopy for Quantitative 3D Analysis of the Developing Ovarian Reserve in Mouse

Published on: September 3, 2021

4.9K
Orthotopic Ovarian Transplantation Procedures to Investigate the Life- and Health-span Influence of Ovarian Senescence in Female Mice
06:49

Orthotopic Ovarian Transplantation Procedures to Investigate the Life- and Health-span Influence of Ovarian Senescence in Female Mice

Published on: February 12, 2018

11.1K

Area of Science:

  • Reproductive Biology
  • Endocrinology
  • Cellular Biology

Background:

  • Premature ovarian insufficiency (POI) is a significant concern affecting women's reproductive health.
  • Oxidative stress is implicated in the pathogenesis of POI, but the underlying molecular mechanisms require further elucidation.
  • The role of SIRT1 and mitochondrial function in POI is not fully understood.

Purpose of the Study:

  • To establish an oxidative stress-induced model of POI using 3-nitropropionic acid (3-NPA) in mice.
  • To investigate the impact of SIRT1 and mitochondrial oxidative phosphorylation (OXPHOS) on ovarian reserve in this model.
  • To explore the therapeutic potential of modulating SIRT1 activity for POI.

Main Methods:

  • An oxidative stress-induced POI model was created in mice using intraperitoneal injections of 3-NPA.
  • SIRT1 expression was analyzed in ovarian tissue.
  • SIRT1 knockdown was performed in human granulosa cells (GCs) to assess its function.
  • SIRT1 antagonist (Ex-527) and agonist (resveratrol) were used to evaluate effects on ovarian function and OXPHOS in vivo.

Main Results:

  • A 40 mg/kg 3-NPA dose for 3 weeks effectively induced POI with decreased SIRT1 expression.
  • SIRT1 knockdown in GCs impaired estrogen synthesis and related gene expression.
  • 3-NPA and Ex-527 reduced ovarian function and inhibited OXPHOS, while resveratrol improved ovarian function and OXPHOS.
  • Apoptosis-related proteins (P53, CASPASE 3, BAX) were altered, with BCL2 showing opposite trends.

Conclusions:

  • 3-NPA administration effectively models POI, characterized by increased oxidative stress, reduced SIRT1, and impaired mitochondrial OXPHOS.
  • These molecular changes contribute to follicular apoptosis and ovarian reserve depletion.
  • Targeting SIRT1, particularly with agonists like resveratrol, shows promise for restoring ovarian function in POI.