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Published on: December 18, 2017
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Xenogeneic-Free Human Intestinal Organoids for Assessing Intestinal Nutrient Absorption
Makoto Inoue1, Yuichi Tanaka1, Sakiko Matsushita1
1Research & Business Development Center, Converting Business Development Unit, Dai Nippon Printing Co., 250-1, Wakashiba, Kashiwa 277-0871, Chiba, Japan.
Nutrients
|March 12, 2022
Summary
Xenogeneic-free human intestinal organoids (XF-HIOs) accurately model nutrient absorption, showing transporter dependence for glucose, dipeptides, and cholesterol. This method effectively screens functional food ingredients, identifying epicatechin gallate and nobiletin as glucose absorption inhibitors.
Area of Science:
- Gastroenterology and Nutrition Science
- In Vitro Modeling
- Nutrient Transport Mechanisms
Background:
- Accurate assessment of nutrient absorption is crucial for understanding food effects on intestinal tissue.
- Current in vitro models like Caco-2 cells and animal models lack human physiological relevance.
- A need exists for advanced in vitro methods to analyze intestinal absorption and digestion more precisely.
Purpose of the Study:
- To validate xenogeneic-free human intestinal organoids (XF-HIOs) as a superior in vitro model for nutrient absorption studies.
- To investigate the transporter-dependent absorption of glucose, dipeptides, and cholesterol in XF-HIOs.
- To evaluate the inhibitory effects of natural compounds on nutrient absorption in XF-HIOs.
Main Methods:
- Utilized three-dimensional xenogeneic-free human intestinal organoids (XF-HIOs) that mimic human intestinal characteristics.
- Assessed the absorption of glucose, dipeptides, and cholesterol by XF-HIOs, confirming transporter dependency.
- Screened natural ingredients, including epicatechin gallate, nobiletin, luteolin, and quercetin, for their impact on glucose and cholesterol absorption.
Main Results:
- XF-HIOs demonstrated transporter-dependent absorption of glucose, dipeptides, and cholesterol.
- Epicatechin gallate (from green tea) and nobiletin (from citrus fruits) significantly suppressed glucose absorption.
- Luteolin and quercetin (from vegetables) did not inhibit cholesterol absorption in this model.
Conclusions:
- XF-HIOs provide a physiologically relevant platform for studying nutrient absorption and transporter function.
- This model is effective for screening the absorption and inhibitory effects of functional food substances.
- The findings support the utility of XF-HIOs for evaluating the bioavailability and efficacy of dietary components.
