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Related Concept Videos

Effects of EDTA on End-Point Detection Methods01:18

Effects of EDTA on End-Point Detection Methods

367
Different methods, such as visual observance of metal-ion indicators, spectroscopic techniques, and potentiometric methods, can determine the endpoint of an EDTA titration.
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a...
367

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Updated: Sep 30, 2025

Sampling and Pretreatment of Tooth Enamel Carbonate for Stable Carbon and Oxygen Isotope Analysis
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Tooth decalcification using different decalcifying agents - A comparative study.

Amberpreet Kaur Khangura1, Shally Gupta1, Anubha Gulati1

  • 1Department of Oral Pathology & Microbiology, Dr. Harvansh Singh Judge Institute of Dental Sciences and Hospital, Panjab University, Chandigarh, India.

Journal of Oral and Maxillofacial Pathology : JOMFP
|March 14, 2022
PubMed
Summary
This summary is machine-generated.

Five percent trichloroacetic acid is the most effective decalcifying agent, balancing tissue integrity and processing time for routine histopathology. This study compared six agents for mineralized tissue processing.

Keywords:
Decalcificationdecalcifying agentshistopathology

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Area of Science:

  • Histopathology
  • Mineralized Tissue Processing
  • Microscopic Assessment

Background:

  • Decalcification is a critical step in microscopic assessment of mineralized tissues.
  • Evaluating the efficacy of different decalcifying agents is essential for optimal tissue processing.

Purpose of the Study:

  • To compare the decalcification time of six chemical agents.
  • To assess the impact of decalcifying agents on cellular and nuclear morphology.
  • To evaluate staining intensity and determine the ideal decalcification technique.

Main Methods:

  • Six decalcifying agents were tested on 30 human permanent teeth.
  • Decalcification endpoint was verified using chemical and radiographic methods.
  • Specimens were processed, sectioned, stained with Hematoxylin and Eosin, and microscopically graded.

Main Results:

  • Perenyi's fluid and 5% trichloroacetic acid offered the fastest decalcification; 14% EDTA was slowest.
  • 5% Trichloroacetic acid, 8% formic acid, formalin-nitric acid, and 5% nitric acid facilitated easier sectioning.
  • Optimal structural details and staining were observed with 5% trichloroacetic acid and neutral EDTA.

Conclusions:

  • 5% Trichloroacetic acid demonstrated the most efficient results, balancing tissue integrity and time.
  • This agent is suitable for routine histopathological diagnosis due to its stability and efficacy.