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Updated: Sep 12, 2025
Characterization of Leukocyte-platelet Rich Fibrin, A Novel Biomaterial
Published on: September 29, 2015
A Comparative Evaluation of Platelet-Rich Fibrin and Platelet-Rich Fibrin Matrix in Terms of pH, Antimicrobial
Jasmine1, Hemant Batra1, Vandana Chhabra1
1Department of Oral & Maxillofacial Surgery, Dr. Harvansh Singh Judge Institute of Dental Sciences & Hospital, Panjab University, Chandigarh, India.
Background:
In recent times, autologous platelet concentrates (APCs) like platelet-rich fibrin (PRF) and platelet-rich fibrin matrix (PRFM) are utilized broadly to promote soft and hard tissue regeneration. The aim of the study was to evaluate PRF and PRFM in terms of pH changes (over a five-day period), antimicrobial potential and histological presentation using light microscopy and field-emission scanning electron microscopic analysis (FESEM).
Materials And Methods:
A prospective comparative in vitro study was conducted in which PRF and PRFM samples were prepared from 21 healthy volunteers and a comparative evaluation for the parameters was carried out.
Results:
A statistically significant difference was found between PRF and PRFM in terms of pH over a five-day period; antimicrobial efficacy at 48 h; average thickness of fibrin; distribution of platelets and leucocytes entrapped in fibrin matrix.
Conclusion:
From our research study, it can be concluded that PRF when compared to PRFM showed abundant entrapment of platelets and leucocytes enmeshed in the 3-D fibrin matrix which is responsible for its better antimicrobial property. PRF also showed increasing acidity over days which accelerates the process of wound healing at the injury site. Thus, PRF is a better autologous platelet concentrate than PRFM.
