Related Experiment Video

Updated: Sep 30, 2025

Nucleocapsid Annealing-Mediated Electrophoresis NAME Assay Allows the Rapid Identification of HIV-1 Nucleocapsid Inhibitors
08:33

Nucleocapsid Annealing-Mediated Electrophoresis NAME Assay Allows the Rapid Identification of HIV-1 Nucleocapsid Inhibitors

Published on: January 19, 2015

9.0K

A host non-coding RNA, nc886, plays a pro-viral role by promoting virus trafficking to the nucleus

Enkhjin Saruuldalai1, Jiyoung Park2, Dongmin Kang2

  • 1Department of Cancer Biomedical Science, Graduate School of Cancer Science and Policy, National Cancer Center, Goyang 10408, Korea.

Molecular Therapy Oncolytics
|March 14, 2022
PubMed

Insights

Human nc886 RNA enhances adenovirus replication by aiding nuclear entry, independent of PKR inhibition. This finding could improve oncolytic virotherapy and create better animal models.

Area of Science:

  • Virology
  • Molecular Biology
  • Cancer Research

Background:

  • Understanding virus-host interactions is key for therapeutic applications.
  • Oncolytic virotherapy using adenovirus shows promise but faces limitations in efficacy and preclinical modeling.
  • Human non-coding RNA nc886 inhibits Protein Kinase R (PKR), an antiviral protein.

Purpose of the Study:

  • To investigate the role of nc886 in adenoviral gene expression and replication.
  • To elucidate the mechanism by which nc886 affects adenovirus.
  • To explore the potential of nc886 in improving oncolytic virotherapy and animal models.

Main Methods:

  • Studied the effect of nc886 on adenoviral gene expression and replication.
  • Investigated the mechanism of nc886's action, including its effect on PKR and nuclear entry.
  • Examined nc886's role in xenogeneic mouse models.

Main Results:

  • nc886 significantly promotes adenoviral gene expression and replication.
  • The pro-adenoviral effect of nc886 is independent of its PKR inhibitory function.
  • nc886 facilitates adenovirus nuclear entry by modulating the kinesin pathway.
  • nc886 promotes adenovirus replication in mouse cells when expressed xenogeneically.

Conclusions:

  • Discovered a novel mechanism where host ncRNA nc886 promotes adenovirus replication via the kinesin pathway.
  • nc886 expression levels may impact oncolytic virotherapy efficacy in certain cancers.
  • nc886 offers potential for enhancing oncolytic adenovirus efficacy and developing improved preclinical models.
Keywords:
Protein Kinase Radenovirushost tropismkinesinnc886non-coding RNAoncolytic virotherapy

More Related Videos

Identification of Nucleolar Factors During HIV-1 Replication Through Rev Immunoprecipitation and Mass Spectrometry09:38

Identification of Nucleolar Factors During HIV-1 Replication Through Rev Immunoprecipitation and Mass Spectrometry

Published on: June 26, 2019

8.2K
Functional Imaging of Viral Transcription Factories Using 3D Fluorescence Microscopy09:03

Functional Imaging of Viral Transcription Factories Using 3D Fluorescence Microscopy

Published on: January 18, 2018

7.2K

Related Concept Videos

Viruses with RNA Genomes01:29

Viruses with RNA Genomes

RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...
183
Types of RNA01:20

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
6.7K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
9.0K
Regulation of Nuclear Protein Sorting01:45

Regulation of Nuclear Protein Sorting

Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
2.5K
Leaky Scanning02:28

Leaky Scanning

During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.3K
Retrovirus Life Cycles01:10

Retrovirus Life Cycles

Retroviruses have a single-stranded RNA genome that undergoes a special form of replication. Once the retrovirus has entered the host cell, an enzyme called reverse transcriptase synthesizes double-stranded DNA from the retroviral RNA genome. This DNA copy of the genome is then integrated into the host’s genome inside the nucleus via an enzyme called integrase. Consequently, the retroviral genome is transcribed into RNA whenever the host’s genome is transcribed, allowing the...
47.2K