Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

A search for new group-B streptococcal serotypes.

J Motlová, M Wagner, J Jelínková

Journal of Medical Microbiology

|September 1, 1986

Summary

Researchers identified new group B Streptococcus (GBS) serotypes in clinical isolates using a sialic acid-specific lectin. This method successfully detected novel GBS types, improving GBS serotyping capabilities.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Arrangements of human telomere DNA quadruplex in physiologically relevant K+ solutions.

Nucleic acids research·2014

Same author

Multilocus sequence types in Czech neonatal GBS strains from 2004 to 2008.

Epidemiologie, mikrobiologie, imunologie : casopis Spolecnosti pro epidemiologii a mikrobiologii Ceske lekarske spolecnosti J.E. Purkyne·2010

Same author

Arrangements of human telomere DNA quadruplex in physiologically relevant K+ solutions.

Nucleic acids research·2009

Same author

Incidence of invasive pneumococcal disease in the Czech Republic and serotype coverage by vaccines, 1997-2006.

Epidemiology and infection·2008

Same author

[Invasive pneumococcal disease in the Czech Republic in 2000-2006].

Epidemiologie, mikrobiologie, imunologie : casopis Spolecnosti pro epidemiologii a mikrobiologii Ceske lekarske spolecnosti J.E. Purkyne·2008

Same author

Emergence of a novel macrolide-resistant Streptococcus pyogenes emm53 strain.

Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2007

Area of Science:

Microbiology
Immunology
Biochemistry

Background:

Group B Streptococcus (GBS) causes significant neonatal infections.
Conventional serotyping of GBS relies on specific antisera for known types (Ia, Ib, II, III).
A subset of clinical GBS isolates remains untypable with existing antisera, necessitating new typing methods.

Purpose of the Study:

To identify novel GBS serotypes among clinical isolates untypable by conventional antisera.
To investigate the role of sialic acid in GBS type polysaccharides.
To evaluate a sialic acid-specific lectin for GBS serotyping.

Main Methods:

Tested 165 untypable GBS clinical isolates for sialic acid presence.
Utilized a sialic acid-specific lectin from *Cepaea hortensis* for slide-agglutination tests on trypsin-treated bacteria.
Confirmed sialic acid detection using chemical measurement (Aminoff's method).

Main Results:

Sialic acid was detected in 96 of 165 isolates.
Ninety-five of these sialic acid-positive strains yielded new serotypes (IV, V, NT6, 7271).
Isolates lacking sialic acid did not possess polysaccharide type-antigens, and 69 strains were negative for sialic acid.

Conclusions:

A sialic acid-specific lectin is effective for identifying novel GBS serotypes.
Sialic acid is a key component of GBS type-specific polysaccharides.
This approach enhances GBS serotyping and epidemiological surveillance.

Related Experiment Videos