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Updated: Sep 30, 2025
Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples
Published on: May 4, 2012
Biological variation and reference change values of serum Mac-2-binding protein glycosylation isomer (M2BPGi)
Rihwa Choi1,2, Gayoung Chun3, Unyeong Go3
1Department of Laboratory Medicine, Green Cross Laboratories, Yongin, Republic of Korea.
Background:
Limited data are available with regard to biological variations of the Mac-2-binding protein glycosylation isomer (M2BPGi), a liver fibrosis biomarker.
Methods:
Long-term biological variation of M2BPGi was investigated using longitudinally measured M2BPGi test results from healthy Korean adult subjects. One-way analysis of variance (ANOVA) tests were used to calculate the reference change value (RCV) of M2BPGi based on biological variation estimates. Furthermore, asymmetric RCV was calculated according to a recent publication of the European Federation of Clinical Chemistry and Laboratory Medicine Working Group on Biological Variation and Task Group for the Biological Variation Database (EFLM TG-BVD).
Results:
A total of 363 test results from 174 Korean subjects undergoing general health checkups were requested from 13 local clinics and hospitals during a 38-month period. The within-subjects biological variation (CVI ), between-subject biological variation (CVG ), analytical variation (CVA ), RCV, and individuality index (II) values for serum M2BPGi were 23.3%, 30.0%, 4.3%, 65.6%, and 0.78, respectively. Asymmetric RCV calculated using formulae by a recent EFLM TG-BVD publication ranged from -41.9 to 72.0%. Desirable analytical performance specifications for M2BPGi derived from biological variation were as follows: imprecision 11.6%, bias 9.6%, and total allowable error 28.7%.
Conclusions:
RCV based on biological estimates may be helpful for evaluating and interpreting serial M2BPGi measurements by physicians and in clinical laboratories.
