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Published on: March 15, 2019
The Effect of Ultrasonic Liposuction Energy Levels on Fat Graft Viability
İbrahim Giray Genç1, Kemal Fındıkçıoğlu2, Ali Sadioğlu2
1Department of Plastic, Reconstructive and Aesthetic Surgery, Faculty of Medicine, Gazi University, 06500, Ankara, Turkey. giraygenc1@gmail.com.
Background:
The use of fat obtained from ultrasound-assisted liposuction is popular. However, no study has considered the effect of different energy levels on fat grafts.
Objectives:
We hypothesized that different ultrasonic energy levels could change the fat graft viability.
Methods:
Both flanks of 15 CD1 nude mice (30 experimental areas) were used, with experimental areas randomly distributed into five groups. Using different energy settings, fat grafts were obtained from a patient's abdominoplasty material and applied to the mouse flank regions. Device settings were intermittent mode with 50% vibration amplitude in group 1, continuous mode with 50% vibration amplitude in group 2, intermittent mode with 90% vibration amplitude in group 3, and continuous mode with 90% vibration amplitude in group 4. The control group was grafted with fat obtained via the conventional method. After 6 weeks, all mice were sacrificed, and fat grafts were excised. Sections were stained with hematoxylin-eosin, Masson's trichrome, and anti-perilipin A antibody.
Results:
The perilipin A immunostaining result was lowest in group 4, indicating the lowest viable cell count (p < 0.01). There was no significant difference between groups for the other parameters (p > 0.05).
Conclusion:
High ultrasonic energy may affect fat graft survival. If fat injection is planned, avoiding high energy settings (our recommendation is not to exceed 16 Watts.) should be considered. We also recommend increasing the vibration amplitude rather than switching from intermittent to continuous mode in body parts that are relatively resistant to liposuction.
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