Related Experiment Video
Updated: Sep 30, 2025
08:51
Preparation of Polypentafluorophenyl acrylate Functionalized SiO2 Beads for Protein Purification
Published on: November 19, 2018
9.8K
Electrically Switchable Polymer Brushes for Protein Capture and Release in Biological Environments.
Gustav Ferrand-Drake Del Castillo1, Maria Kyriakidou1, Zeynep Adali1
1Department of Chemistry and Chemical Engineering, Chalmers University of Technology, Kemigården 4, 41296, Göteborg, Sweden.
Angewandte Chemie (International Ed. in English)
|March 15, 2022
Summary
Researchers developed an electrically switchable interface that prevents protein adsorption and binds proteins on demand. This technology enables on-demand non-fouling and controlled protein interactions in biological systems.
Area of Science:
- Biomaterials Science
- Surface Chemistry
- Biotechnology
Background:
- Polymer-functionalized interfaces offer protein resistance and binding capabilities.
- Switching between these states typically requires environmental changes, limiting biological applications.
Purpose of the Study:
- To develop an interface with electrically controlled switching between protein binding and non-fouling states.
- To demonstrate the interface's functionality in biological fluids and its potential for various applications.
Main Methods:
- Fabrication of polymer-functionalized interfaces.
- Electrical stimulation for switching between protein binding and non-fouling states.
- Characterization of protein adsorption and desorption dynamics.
Main Results:
- The interface demonstrated reversible switching between a high-capacity protein binding state (>1 μg/cm²) and a non-fouling state.
- Switching was achieved over multiple cycles without regeneration, even in biological fluids.
- No detectable protein adsorption occurred in the non-fouling state.
Conclusions:
- This novel interface offers on-demand control over protein interactions.
- The technology has significant potential for applications in biosensing, separations, and in vivo drug delivery.
