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Updated: Sep 30, 2025
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A Sensitive Visual Method for the Detection of Hydrogen Sulfide Producing Bacteria
Published on: June 27, 2022
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An algorithm-assisted automated identification and enumeration system for sensitive hydrogen sulfide sensing under
Shaohua Xu1,2, Xiaoyu Deng3, Shuyi Ji4
1Jiangxi Engineering Research Centre for Translational Cancer Technology, Jiangxi University of Chinese Medicine, Nanchang, Jiangxi, 330004, China.
The Analyst
|March 15, 2022
Summary
A new method uses gold nanoparticles and dark field microscopy to detect hydrogen sulfide (H2S) levels. This sensitive approach accurately measures H2S in biological samples, offering a promising tool for research.
Area of Science:
- Biochemistry
- Nanotechnology
- Analytical Chemistry
Background:
- Hydrogen sulfide (H2S) is a crucial signaling molecule in physiological processes.
- Accurate detection of intracellular H2S is vital for understanding life functions.
Purpose of the Study:
- To develop a sensitive and effective method for H2S sensing.
- To utilize dark field microscopy (DFM) and gold nanoparticles (AuNPs) for H2S detection.
Main Methods:
- AuNPs modified with azido and alkynyl were used as signal reporters.
- H2S selectively reduced azido to amino, inhibiting click chemistry and controlling AuNP aggregation.
- DFM visualized and an algorithm quantified single AuNPs versus aggregated AuNPs.
Main Results:
- The method achieved linear H2S analysis in the range of 2-80 μM.
- The sensing strategy was successfully applied to detect H2S in cell lysate.
- Results showed no significant difference compared to traditional colorimetric methods.
Conclusions:
- The developed DFM-based method offers sensitive and effective H2S detection.
- The integration of AuNPs, DFM, and an intelligent algorithm shows promise for biological sensing.
- This approach provides a valuable tool for H2S quantification in complex biological samples.
